Western Blot (WB)
(Anti-UBA1 mouse monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:111 kDaObserved band size:100 kDa)
Mouse anti-Human Ubiquitin Activating Enzyme E1/UBA1 Monoclonal Antibody | anti-UBA1 antibody
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody, Mouse Monoclonal
No cross-reactivity in ELISA with Insect cell lysate
ELISA: 1:5000-1:10,000
IP: 0.2-1uL/mg of lysate
Western Blot (WB)
(Anti-UBA1 mouse monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:111 kDaObserved band size:100 kDa)
Western Blot (WB)
(Anti-UBA1 mouse monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:111 kDaObserved band size:100 kDa)
Immunoprecipitation (IP)
(UBA1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate0.5 uL anti-UBA1 mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-UBA1 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa)
Immunoprecipitation (IP)
(UBA1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate0.5 uL anti-UBA1 mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-UBA1 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
UBE1: an enzyme of the ubiquitin-activating E1 family. Adenylates ubiquitin at its carboxy-terminal glycine residue and thereafter links this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester. There are two active sites within the E1 molecule, allowing it to accomodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.
Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: heterochromatin; desmosome; mitochondrion; lysosomal membrane; cytoplasm; endosome membrane; rough endoplasmic reticulum membrane; nucleus; cytosol
Molecular Function: protein binding; ubiquitin-protein ligase activity; ubiquitin activating enzyme activity; ATP binding
Biological Process: modification-dependent protein catabolic process; protein ubiquitination; response to DNA damage stimulus
Disease: Spinal Muscular Atrophy, X-linked 2
Research Articles on UBA1
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Product Notes
The UBA1 uba1 (Catalog #AAA8106080) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin Activating Enzyme E1/UBA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the UBA1 uba1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin Activating Enzyme E1/UBA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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