Rabbit anti-Human NOD1 Polyclonal Antibody | anti-NOD1 antibody

Polyclonal Antibody to NOD1

Cat. Num. AAA668736

• Gene Names: NOD1; CARD4; NLRC1; CLR7.1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Serum
• Synonyms: NOD1; Polyclonal Antibody; Polyclonal Antibody to NOD1; CARD4; Caspase recruitment domain-containing protein 4; Antibody to NOD1; Anti-NOD1 Antibody; NOD1 Antibody; anti-NOD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Serum
• Form/Format: 50 ul sera
• Sequence Length: 953

• Applicable Applications for anti-NOD1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
• Application Notes: WB: 1: 1000-1: 2000; IHC (paraffin): 1: 1000-1: 5000; IP: 1: 50-1: 200
• Immunogen Information: A synthetic peptide of human NOD1 (amino acids 494-512 QLGFLRALPELGPGGDQQS) was used as immunogen for this antibody
• Antigen: NOD1
• Preparation and Storage: Store the antibody at 4 degree C, stable for 6 months.; For long-term storage, store at -20 degree C.; Avoid repeated freeze and thaw cycles.

Immunohistochemistry (IHC)

(Fig: 1 Formalin-fixed, paraffin-embedded section of human sebaceous gland (A) and colon (B) stained for NOD1 expression using NOD1 antibody at 1: 2000. Hematoxylin counterstain. 10X magnification)

Immunohistochemistry (IHC)

(Fig: 2 Formalin-fixed, paraffin-embedded sections of human colon (A, A1) and pancreas (B, B1) stained for NOD1 expression (NOD1 antibody at 1: 2000) using a DAB chromogen and Hematoxylin counterstain. Magnification: A (20X), A1 (40X). B (10X), B1 (40X).)

Related Product Information for anti-NOD1 antibody

This antibody recognizes NOD1 (CARD4). Human NOD1 (CARD4) is a 953 amino acid protein. NOD (nucleotide-binding oligomerization domain) proteins are a family of cytosolic proteins which have been implicated in innate recognition of bacteria, the induction of inflammatory responses, and the regulation of caspase activation and apoptosis. NOD1/CARD4 (caspase-recruitment domain 4 gene) is a PRM that recognizes specific peptidoglycan (PGN) components of bacterial cell walls. NOD1 is expressed by cell types that are exposed to PGN under physiological conditions including antigen-presenting cells (APCs) such as macrophages and dendritic cells, and epithelial cells (reviewed in Strober et al. 2006). NOD1 is thought to play a role in the pathogenesis of human gastrointestinal disease and is associated with inflammatory bowel diseases and asthma. It is involved in host defense against Helicobacter pylori infection of the gastric mucosa, a chronic infection that can lead to peptic ulcers and gastric cancer.

Product Categories/Family for anti-NOD1 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 20137579
• NCBI GeneID: 10392
• UniProt Accession #: Q9Y239; Q549U4; Q8IWF5; B4DTU3
• Molecular Weight: 27,949 Da
• NCBI Official Full Name: Nucleotide-binding oligomerization domain-containing protein 1
• NCBI Official Synonym Full Names: nucleotide binding oligomerization domain containing 1
• NCBI Official Symbol: NOD1
• NCBI Official Synonym Symbols: CARD4; NLRC1; CLR7.1
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 1
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 1
• Protein Family: Nucleotide-binding oligomerization domain-containing protein
• UniProt Gene Name: NOD1
• UniProt Synonym Gene Names: CARD4

NCBI Description

This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Oct 2009]

Uniprot Description

NOD1: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: basolateral plasma membrane; cytoplasm; cytosol

Molecular Function: CARD domain binding; caspase activator activity; identical protein binding; peptidoglycan binding; protein binding; protein homodimerization activity

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; caspase activation; defense response; defense response to bacterium; detection of bacterium; detection of biotic stimulus; inflammatory response; interleukin-8 biosynthetic process; JNK cascade; positive regulation of caspase activity; positive regulation of dendritic cell antigen processing and presentation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein oligomerization; signal transduction