Rabbit anti-Human MIZF Polyclonal Antibody | anti-MBD2 antibody

MIZF (MBD2 Interacting Zinc Finger Protein, Methyl CpG Binding Protein 2 Interacting Zinc Finger Protein, MBD2 (Methyl-CpG-Binding Protein)-Interacting Zinc Finger Protein)

Cat. Num. AAA613654

• Gene Names: MBD2; DMTase; NY-CO-41; DKFZp586O0821
• Reactivity: Human
• Applications: Western Blot
• Purity: Serum; Serum
• Synonyms: MIZF; Polyclonal Antibody; MIZF (MBD2 Interacting Zinc Finger Protein; Methyl CpG Binding Protein 2 Interacting Zinc Finger Protein; MBD2 (Methyl-CpG-Binding Protein)-Interacting Zinc Finger Protein); Anti -MIZF (MBD2 Interacting Zinc Finger Protein; anti-MBD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MBD2-binding zinc finger (MIZF).
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in 0.02% sodium azide.

• Applicable Applications for anti-MBD2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500 detects a band of ~64kD.
• Immunogen: Synthetic peptides corresponding to aa residues, 180-194, 331-346, and 371-388 of human MIZF.
• Positive Control: MOLT-4 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of MIZF using MOLT-4 cell lysate; 10ug of protein was loaded per lane; MBS613654 was used at 1:500 dilution.)

Related Product Information for anti-MBD2 antibody

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleavage of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 were later found to co-localize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. Recently, using MBD2 as bait in two-hybrid screening, a novel zinc finger protein, called MIZF has been identified (3). This MBD2-binding zinc finger (MIZF) protein represses transcription by associating with MBD2 and a histone deacetylase complex. It is ubiquitously expressed in all human tissues.

Product Categories/Family for anti-MBD2 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 4505117
• NCBI GeneID: 8932
• NCBI Accession #: NP_003918.1
• NCBI GenBank Nucleotide #: NM_003927.4
• UniProt Accession #: Q9UBB5; O95242; Q9UIS8; O60535; Q69YZ5
• Molecular Weight: 43,255 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 2 isoform 1
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 2
• NCBI Official Symbol: MBD2
• NCBI Official Synonym Symbols: DMTase; NY-CO-41; DKFZp586O0821
• NCBI Protein Information: methyl-CpG-binding domain protein 2; demethylase; OTTHUMP00000163558
• UniProt Protein Name: Methyl-CpG-binding domain protein 2
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD2
• UniProt Entry Name: MBD2_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

MBD2: Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. May enhance the activation of some unmethylated cAMP-responsive promoters. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: nucleoplasm; heterochromatin; protein complex; histone deacetylase complex; nuclear chromatin; cytoplasm; nucleus

Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; nucleosomal DNA binding; methyl-CpG binding; satellite DNA binding

Biological Process: cellular protein complex assembly; transcription, DNA-dependent; maternal behavior; negative regulation of gene expression, epigenetic; ATP-dependent chromatin remodeling; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic; positive regulation of Wnt receptor signaling pathway; regulation of cell proliferation