Sheep anti-Human NMNAT1 Polyclonal Antibody | anti-NMNAT1 antibody

NMNAT1 (Nicotinamide Mononucleotide Adenylyltransferase 1, NMN Adenylyltransferase 1)

Cat. Num. AAA626472

• Gene Names: NMNAT1; LCA9; NMNAT; PNAT1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NMNAT1; Polyclonal Antibody; NMNAT1 (Nicotinamide Mononucleotide Adenylyltransferase 1; NMN Adenylyltransferase 1); Anti -NMNAT1 (Nicotinamide Mononucleotide Adenylyltransferase 1; anti-NMNAT1 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NMNAT-1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

• Applicable Applications for anti-NMNAT1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Immunoprecipitation, and Western Blot.; Dilution: ELISA: 0.5-1ug/ml; Western Blot: 1ug/ml
• Immunogen: Purified, Sf 21-derived, recombinant human NMNAT-1 (aa4-279).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of NMAT-1: Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-hNMNAT-1.)

Related Product Information for anti-NMNAT1 antibody

Axonal degeneration contributes to the pathogenesis of several neurodegenerative states following mechanical or neurotoxic injury. Several studies have now demonstrated that overexpression of Nmnat1 (nicotinamide mononucleotide adenylyltransferase), a ubiquitously expressed nuclear enzyme that catalyzes an essential step in the NAD (NADP) biosynthetic pathway, has the ability to protect axons from degeneration following traumatic injury.

Product Categories/Family for anti-NMNAT1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 20070321
• NCBI GeneID: 64802
• NCBI Accession #: NP_073624.2
• NCBI GenBank Nucleotide #: NM_022787.3
• UniProt Accession #: Q9HAN9; Q8TAE9; Q9H247; Q9H6B6; B1AN63
• Molecular Weight: 31,932 Da
• NCBI Official Full Name: nicotinamide mononucleotide adenylyltransferase 1
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 1
• NCBI Official Symbol: NMNAT1
• NCBI Official Synonym Symbols: LCA9; NMNAT; PNAT1
• NCBI Protein Information: nicotinamide mononucleotide adenylyltransferase 1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1; pyridine nucleotide adenylyltransferase 1; nicotinate-nucleotide adenylyltransferase 1
• UniProt Protein Name: Nicotinamide mononucleotide adenylyltransferase 1
• UniProt Gene Name: NMNAT1
• UniProt Synonym Gene Names: NMNAT; NMN adenylyltransferase 1
• UniProt Entry Name: NMNA1_HUMAN

NCBI Description

This gene encodes an enzyme which catalyzes a key step in the biosynthesis of the coenzyme NAD. The encoded protein is one of several nicotinamide nucleotide adenylyltransferases. Studies in Drosophila and mammalian neurons have shown the encoded protein can confer protection to damaged neurons. This protection requires enzymatic activity which increases NAD levels and activates a nuclear deacetylase which is the protective molecule. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14 and 15. [provided by RefSeq, Dec 2011]

Uniprot Description

NMNAT1: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following mechanical or toxic insults. Belongs to the eukaryotic NMN adenylyltransferase family.

Protein type: Transferase; EC 2.7.7.1; EC 2.7.7.18; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; ATP binding

Biological Process: NAD biosynthetic process; vitamin metabolic process; NAD metabolic process; water-soluble vitamin metabolic process

Disease: Leber Congenital Amaurosis 9