Nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1) Recombinant Protein | NMNAT1 recombinant protein

Recombinant Human Nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1)

Cat. Num. AAA1387855

• Gene Names: NMNAT1; LCA9; NMNAT; PNAT1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1); Recombinant Human Nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1); Nicotinamide mononucleotide adenylyltransferase 1; NMN adenylyltransferase 1; EC=2.7.7.1; Nicotinate-nucleotide adenylyltransferase 1; NaMN adenylyltransferase 1; EC=2.7.7.18; NMNAT1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-279aa; Full Length
• Sequence: MENSEKTEVVLLACGSFNPITNMHLRLFELAKDYMNGTGRYTVVKGIISPVGDAYKKKGLIPAYHRVIMAELATKNSKWVEVDTWESLQKEWKETLKVLRHHQEKLEASDCDHQQNSPTLERPGRKRKWTETQDSSQKKSLEPKTKAVPKVKLLCGADLLESFAVPNLWKSEDITQIVANYGLICVTRAGNDAQKFIYESDVLWKHRSNIHVVNEWIANDISSTKIRRALRRGQSIRYLVPDLVQEYIEKHNLYSSESEDRNAGVILAPLQRNTAEAKT
• Sequence Length: 279

• Species: Homo sapiens (Human)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

SDS-PAGE

Related Product Information for NMNAT1 recombinant protein

Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, prefers NAD+ and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NaADP+. Protects against axonal degeneration following mechanical or toxic insults

Product Categories/Family for NMNAT1 recombinant protein

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 20070321
• NCBI GeneID: 64802
• NCBI Accession #: NP_073624.2
• NCBI GenBank Nucleotide #: NM_022787.3
• UniProt Accession #: Q9HAN9; Q8TAE9; Q9H247; Q9H6B6; B1AN63
• Molecular Weight: 58.9 kDa
• NCBI Official Full Name: nicotinamide mononucleotide adenylyltransferase 1
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 1
• NCBI Official Symbol: NMNAT1
• NCBI Official Synonym Symbols: LCA9; NMNAT; PNAT1
• NCBI Protein Information: nicotinamide mononucleotide adenylyltransferase 1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1; Leber's congenital amaurosis 9; pyridine nucleotide adenylyltransferase 1; nicotinate-nucleotide adenylyltransferase 1
• UniProt Protein Name: Nicotinamide mononucleotide adenylyltransferase 1
• UniProt Gene Name: NMNAT1
• UniProt Synonym Gene Names: NMNAT; NMN adenylyltransferase 1; NaMN adenylyltransferase 1
• UniProt Entry Name: NMNA1_HUMAN

NCBI Description

This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15. [provided by RefSeq, Jul 2014]

Uniprot Description

NMNAT1: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following mechanical or toxic insults. Belongs to the eukaryotic NMN adenylyltransferase family.

Protein type: EC 2.7.7.1; EC 2.7.7.18; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; ATP binding

Biological Process: NAD biosynthetic process; vitamin metabolic process; NAD metabolic process; water-soluble vitamin metabolic process

Disease: Leber Congenital Amaurosis 9