Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: PDE9A antibody at 3.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 69, 62, 55, 63, 58, 46, 59, 51, 45, 54, 62, 65, 66, 57 KDaObserved band size: 69 KDa)
Rabbit anti-Human PDE9A Polyclonal Antibody | anti-PDE9A antibody
PDE9A Antibody
IHC: 1:500-1:1000
IF: 1:50-1:200
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: PDE9A antibody at 3.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 69, 62, 55, 63, 58, 46, 59, 51, 45, 54, 62, 65, 66, 57 KDaObserved band size: 69 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: PDE9A antibody at 3.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 69, 62, 55, 63, 58, 46, 59, 51, 45, 54, 62, 65, 66, 57 KDaObserved band size: 69 KDa)
Immunohistochemistry (IHC)
(IHC image of MBS7107191 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7107191 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(Immunofluorescence staining of A549 cells with MBS7107191 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunohistochemistry (IHC)
(Immunofluorescence staining of A549 cells with MBS7107191 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP (PubMed:9624146, PubMed:18757755, PubMed:21483814). Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed:25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory ().
Research Articles on PDE9A
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Product Notes
The PDE9A pde9a (Catalog #AAA7107191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE9A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDE9A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:5000 IHC: 1:500-1:1000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the PDE9A pde9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE9A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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