Rabbit anti-Human MAGEA6 Polyclonal Antibody | anti-MAGEA6 antibody

MAGEA6, CT (Melanoma-associated Antigen 6, MAGE-6 Antigen, MAGE-3B Antigen, Cancer/Testis Antigen 1.6, CT1.6, MAGE6) (FITC)

Cat. Num. AAA6486245

• Gene Names: MAGEA6; CT1.6; MAGE6; MAGE3B; MAGE-3b
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAGEA6; Polyclonal Antibody; CT (Melanoma-associated Antigen 6; MAGE-6 Antigen; MAGE-3B Antigen; Cancer/Testis Antigen 1.6; CT1.6; MAGE6) (FITC); anti-MAGEA6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAGEA6.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MAGEA6 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MAGEA6 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-MAGEA6 C-term Antibody is used in Western blot to detect MAGEA6 in A549 lysate.)

Product Categories/Family for anti-MAGEA6 antibody

Antibodies; Cancer Markers

References

1. Tatsumi, T., et al., Clin. Cancer Res. 9(3):947-954 (2003). 2. Tatsumi, T., et al., J. Exp. Med. 196(5):619-628 (2002). 3. Imai, Y., et al., Gene 160(2):287-290 (1995). 4. Rogner, U.C., et al., Genomics 29(3):725-731 (1995). 5. De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 4885469
• NCBI GeneID: 4105
• NCBI Accession #: NP_005354
• NCBI GenBank Nucleotide #: NM_005363.3
• UniProt Accession #: P43360; Q6NW44; A8IF93
• Molecular Weight: 35.9 kDa (322aa), confirmed by MALDI-TOF
• NCBI Official Full Name: melanoma-associated antigen 6
• NCBI Official Synonym Full Names: MAGE family member A6
• NCBI Official Symbol: MAGEA6
• NCBI Official Synonym Symbols: CT1.6; MAGE6; MAGE3B; MAGE-3b
• NCBI Protein Information: melanoma-associated antigen 6
• UniProt Protein Name: Melanoma-associated antigen 6
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEA6
• UniProt Synonym Gene Names: MAGE6; CT1.6
• UniProt Entry Name: MAGA6_HUMAN

NCBI Description

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

MAGE-A6: Proposed to enhance ubiquitin ligase activity of RING- type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28

Molecular Function: protein binding