Rabbit NLRC3 Polyclonal Antibody | anti-NLRC3 antibody

NLRC3 Antibody

Cat. Num. AAA9612170

• Gene Names: NLRC3; NOD3; CLR16.2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NLRC3; Polyclonal Antibody; NLRC3 Antibody; CARD15-like; CARD15-like protein; Caterpiller 16.2; Caterpiller protein 16.2; CLR16.2; FLJ00348; NLR family CARD domain containing 3; Nlrc3; NLRC3_HUMAN; NOD-like receptor C3; NOD3; NOD3 protein; Nucleotide-binding oligomerization domain protein 3; Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2; nucleotide-binding oligomerization domain; leucine rich repeat and CARD domain containing 3; Protein NLRC3; anti-NLRC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NLRC3 Antibody detects endogenous levels of total NLRC3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1112

• Applicable Applications for anti-NLRC3 antibody: ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ICC: 1:100-1:500; WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human NLRC3, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from 3T3, using NLRC3 Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612170 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612170 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-NLRC3 antibody

Function: Negative regulator of the innate immune response (PubMed:15705585, PubMed:22863753, PubMed:25277106). Attenuates signaling pathways activated by Toll-like receptors (TLRs) and the DNA sensor STING/TMEM173 in response to pathogen-associated molecular patterns, such as intracellular poly(dA:dT), but not poly(I:C), or in response to DNA virus infection, including that of Herpes simplex virus 1 (HSV1) (By similarity) (PubMed:22863753). May affect TLR4 signaling by acting at the level of TRAF6 ubiquitination, decreasing the activating 'Lys-63'-linked ubiquitination and leaving unchanged the degradative 'Lys-48'-linked ubiquitination (PubMed:22863753). Inhibits the PI3K-AKT-mTOR pathway possibly by directly interacting with the posphatidylinositol 3-kinase regulatory subunit p85 (PIK3R1/PIK3R2) and disrupting the association between PIK3R1/PIK3R2 and the catalytic subunit p110 (PIK3CA/PIK3CB/PIK3CD) and reducing PIK3R1/PIK3R2 activation. Via its regulation of the PI3K-AKT-mTOR pathway, controls cell proliferation, predominantly in intestinal epithelial cells (By similarity). May also affect NOD1- or NOD2-mediated NF-kappa-B activation (PubMed:25277106). Might also affect the inflammatory response by preventing NLRP3 inflammasome formation, CASP1 cleavage and IL1B maturation (PubMed:25277106).

Subcellular Location: Cytoplasm.

Subunit Structure: Directly interacts (via CARD) with TMEM173/STING; this interaction reduces TMEM173 trafficking to the perinuclear region in response to interferon stimulatory DNA. Also interacts, but to a lesser extent, with TBK1 (PubMed:24560620). Interacts with TRAF6; this interaction results in decreased TRAF6 'Lys-63'-linked polyubiquitination, but leaves 'Lys-48'-linked chains unchanged, promoting TRAF6 protein degradation (PubMed:22863753). Interacts with PIK3R1/PIK3R2; this interaction disrupts the association between PIK3R1/PIK3R2 and the p110 catalytic subunit PIK3CA/PIK3CB/PIK3CD and reduces PIK3R1/PIK3R2 activation (By similarity). Weakly interacts with PYCARD/ASC. Interacts with CASP1 and CASP5 (PubMed:25277106).

Similarity: The leucine-rich repeat domain may reduce the interaction with TMEM173/STING.Belongs to the NLRP family.

NCBI and Uniprot Product Information

• NCBI GI #: 119605757
• NCBI GeneID: 197358
• UniProt Accession #: Q7RTR2
• Molecular Weight: Observed Molecular Weight: (Observed)114kD.; Predicted Molecular Weight: (Calculated)115kDa.
• NCBI Official Full Name: NOD3 protein, isoform CRA_d
• NCBI Official Synonym Full Names: NLR family CARD domain containing 3
• NCBI Official Symbol: NLRC3
• NCBI Official Synonym Symbols: NOD3; CLR16.2
• NCBI Protein Information: NLR family CARD domain-containing protein 3
• UniProt Protein Name: Protein NLRC3
• Protein Family: Protein
• UniProt Gene Name: NLRC3
• UniProt Synonym Gene Names: NOD3; CLR16.2
• UniProt Entry Name: NLRC3_HUMAN

NCBI Description

This gene encodes a NOD-like receptor family member. The encoded protein is a cytosolic regulator of innate immunity. This protein directly interacts with stimulator of interferon genes (STING), to prevent its proper trafficking, resulting in disruption of STING-dependent activation of the innate immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

NLRC3: May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm

Molecular Function: ATP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; T cell activation; regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; response to lipopolysaccharide; negative regulation of I-kappaB kinase/NF-kappaB cascade