Western Blot (WB)
(CNPY2 Antibody (C-term) western blot analysis in MCF-7,NCI-H460,HepG2,Hela cell line lysates (35ug/lane).This demonstrates the CNPY2 antibody detected the CNPY2 protein (arrow).)
Rabbit anti-Human CNPY2 Polyclonal Antibody | anti-CNPY2 antibody
CNPY2, CT (CNPY2, MSAP, TMEM4, ZSIG9, Protein canopy homolog 2, MIR-interacting saposin-like protein, Putative secreted protein Zsig9, Transmembrane protein 4) (FITC)
Gene Names
CNPY2; MSAP; TMEM4; ZSIG9; HP10390
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CNPY2; Polyclonal Antibody; CT (CNPY2; MSAP; TMEM4; ZSIG9; Protein canopy homolog 2; MIR-interacting saposin-like protein; Putative secreted protein Zsig9; Transmembrane protein 4) (FITC); Transmembrane protein 4; anti-CNPY2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-CNPY2 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CNPY2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 140-170 from the C-terminal region of human CNPY2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(CNPY2 Antibody (C-term) western blot analysis in MCF-7,NCI-H460,HepG2,Hela cell line lysates (35ug/lane).This demonstrates the CNPY2 antibody detected the CNPY2 protein (arrow).)
Western Blot (WB)
(CNPY2 Antibody (C-term) western blot analysis in MCF-7,NCI-H460,HepG2,Hela cell line lysates (35ug/lane).This demonstrates the CNPY2 antibody detected the CNPY2 protein (arrow).)
Flow Cytometry (FC/FACS)
(CNPY2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CNPY2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CNPY2 antibody
CNPY2 is positive regulator of neurite outgrowth by stabilizing myosin regulatory light chain (MRLC). It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation.
Product Categories/Family for anti-CNPY2 antibody
References
Trynka, G., et al. Gut 58(8):1078-1083(2009). Bornhauser, B.C., et al. Cell. Mol. Life Sci. 62(11):1260-1266(2005). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,117 Da
NCBI Official Full Name
protein canopy homolog 2 isoform 2
NCBI Official Synonym Full Names
canopy FGF signaling regulator 2
NCBI Official Symbol
CNPY2
NCBI Official Synonym Symbols
MSAP; TMEM4; ZSIG9; HP10390
NCBI Protein Information
protein canopy homolog 2; canopy 2 homolog; transmembrane protein 4; putative secreted protein Zsig9; MIR-interacting saposin-like protein
UniProt Protein Name
Protein canopy homolog 2
Protein Family
UniProt Gene Name
CNPY2
UniProt Synonym Gene Names
MSAP; TMEM4; ZSIG9
Uniprot Description
Positive regulator of neurite outgrowth by stabilizing myosin regulatory light chain (MRLC). It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation.
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Product Notes
The CNPY2 cnpy2 (Catalog #AAA6287320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CNPY2, CT (CNPY2, MSAP, TMEM4, ZSIG9, Protein canopy homolog 2, MIR-interacting saposin-like protein, Putative secreted protein Zsig9, Transmembrane protein 4) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CNPY2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CNPY2 cnpy2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNPY2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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