Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using FOXF2 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit FOXF2 Polyclonal Antibody | anti-FOXF2 antibody
FOXF2 Antibody
Gene Names
FOXF2; FKHL6; FREAC2; FREAC-2
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
FOXF2; Polyclonal Antibody; FOXF2 Antibody; FKH L6; FKHL 6; FKHL6; Forkhead box F2; Forkhead box protein F2; Forkhead like 6; Forkhead related activator 2; Forkhead related protein FKHL6; Forkhead related transcription factor 2; Forkhead; Drosophila; homolog-like 6; FOX F2; FOXF 2; FREAC 2; FREAC2; OTTHUMP00000017863; anti-FOXF2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
FOXF2 Antibody detects endogenous levels of total FOXF2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
444
Applicable Applications for anti-FOXF2 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20,000-1:40,000
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human FOXF2, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using FOXF2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using FOXF2 Antibody. The lane on the left was treated with blocking peptide.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612378 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612378) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612378 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612378) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Related Product Information for anti-FOXF2 antibody
Function: Probable transcription activator for a number of lung-specific genes (PubMed:8626802). Mediates up-regulation of the E3 ligase IRF2BPL and drives ubiquitination and degradation of CTNNB1 (PubMed:29374064).
Subcellular Location: Nucleus.
Tissue Specificity: Lung and placenta (PubMed:8626802). Predominantly expressed in gastrointestinal tract including stomach (PubMed:29374064).
Subunit Structure: Interacts with the transcription factors TBP and TFIIB.
Similarity: Two activation domains, AD1 and AD2, C-terminal of (and distinct from) the forkhead domains are necessary for transcriptional activation.
Subcellular Location: Nucleus.
Tissue Specificity: Lung and placenta (PubMed:8626802). Predominantly expressed in gastrointestinal tract including stomach (PubMed:29374064).
Subunit Structure: Interacts with the transcription factors TBP and TFIIB.
Similarity: Two activation domains, AD1 and AD2, C-terminal of (and distinct from) the forkhead domains are necessary for transcriptional activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)45kD.
Predicted Molecular Weight: (Calculated)46kDa.
Predicted Molecular Weight: (Calculated)46kDa.
NCBI Official Full Name
forkhead box protein F2
NCBI Official Synonym Full Names
forkhead box F2
NCBI Official Symbol
FOXF2
NCBI Official Synonym Symbols
FKHL6; FREAC2; FREAC-2
NCBI Protein Information
forkhead box protein F2
UniProt Protein Name
Forkhead box protein F2
Protein Family
UniProt Gene Name
FOXF2
UniProt Synonym Gene Names
FKHL6; FREAC2
UniProt Entry Name
FOXF2_HUMAN
NCBI Description
FOXF2 encodes forkhead box F2, one of many human homologues of the Drosophila melanogaster transcription factor forkhead. FOXF2 is expressed in lung and placenta, and has been shown to transcriptionally activate several lung-specific genes. [provided by RefSeq, Jul 2008]
Research Articles on FOXF2
Similar Products
Product Notes
The FOXF2 foxf2 (Catalog #AAA9612378) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FOXF2 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's FOXF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:500 ICC: 1:100-1:500 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the FOXF2 foxf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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