Rabbit PAF1 Polyclonal Antibody | anti-PAF1 antibody

PAF1 Antibody

Cat. Num. AAA9612233

• Gene Names: PAF1; PD2; F23149_1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PAF1; Polyclonal Antibody; PAF1 Antibody; F23149 1; FLJ11123; hPAF1; PAF 1; Paf1 RNA polymerase II associated factor homolog; PAF1_HUMAN; Pancreatic differentiation protein 2; PD 2; PD2; PD2 protein; RNA polymerase II associated factor homolog; RNA polymerase II-associated factor 1 homolog; anti-PAF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PAF1 Antibody detects endogenous levels of total PAF1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 485

• Applicable Applications for anti-PAF1 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human PAF1, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hybridoma cells, using PAF1 Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612233 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612233) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

Related Product Information for anti-PAF1 antibody

Function: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro.

Subcellular Location: Nucleus. Note: Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin.

Subunit Structure: Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61 (PubMed:19952111, PubMed:20178742). The PAF1 complex interacts with PHF5A (By similarity). Interacts with POLR2A, TCEA1, TTC37, KMT2A/MLL1, SUPT5H, RNF20 and RNF40 (PubMed:15923622, PubMed:16024656, PubMed:19952111, PubMed:20178742, PubMed:20541477, PubMed:16491129, PubMed:19410543). Interacts with UBE2E1 (PubMed:16307923). Interacts with influenza A strain H3N2 NS1 protein; the interaction interferes with host cell gene transcription, specifically with that of antiviral genes (PubMed:22419161).

Similarity: Belongs to the PAF1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 378744165
• NCBI GeneID: 54623
• NCBI Accession #: NP_001243755
• NCBI GenBank Nucleotide #: NP_001243755.1
• UniProt Accession #: Q8N7H5
• Molecular Weight: Observed Molecular Weight: (Observed)59kD.; Predicted Molecular Weight: (Calculated)60kDa.
• NCBI Official Full Name: RNA polymerase II-associated factor 1 homolog isoform 2
• NCBI Official Synonym Full Names: PAF1 homolog, Paf1/RNA polymerase II complex component
• NCBI Official Symbol: PAF1
• NCBI Official Synonym Symbols: PD2; F23149_1
• NCBI Protein Information: RNA polymerase II-associated factor 1 homolog
• UniProt Protein Name: RNA polymerase II-associated factor 1 homolog
• Protein Family: Proteasome
• UniProt Gene Name: PAF1
• UniProt Synonym Gene Names: PD2; hPAF1
• UniProt Entry Name: PAF1_HUMAN

NCBI Description

This gene encodes a subunit of the polymerase associated factor (PAF1) complex. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]