Western Blot (WB)
(Western Blot: Sample: Mouse Cerebellum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse NOS2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Nitric Oxide Synthase 2, Inducible (NOS2) Polyclonal Antibody | anti-NOS2 antibody
Polyclonal Antibody to Nitric Oxide Synthase 2, Inducible (NOS2)
MGHHHHHHSGSEF- VMASR VRATVLFATE TGKSEALARD LATLFSYAFN TKVVCMDQYK ASTLEEEQLL LVVTSTFGNG DCPSNGQTLK KSLFMLRELN HTFRYAVFGL GSSMYPQFCA FAHDIDQKLS HLGASQLAPT GEGDELSGQ
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebellum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse NOS2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebellum lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse NOS2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI Description
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Uniprot Description
iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase
Chromosomal Location of Human Ortholog: 11 B5|11 46.74 cM
Cellular Component: cortical cytoskeleton; cytoplasm; cytosol; extracellular space; intracellular; nucleus; perinuclear region of cytoplasm; peroxisome; vesicle membrane
Molecular Function: actin binding; arginine binding; beta-catenin binding; cadherin binding; calmodulin binding; cAMP-dependent protein kinase regulator activity; FAD binding; FMN binding; heme binding; Hsp90 protein binding; iron ion binding; metal ion binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; nitric-oxide synthase binding; oxidoreductase activity; protein binding; protein homodimerization activity; protein kinase binding; receptor binding; tetrahydrobiopterin binding
Biological Process: arginine catabolic process; cellular response to cytokine stimulus; cellular response to interferon-gamma; cellular response to organic cyclic compound; circadian rhythm; defense response to bacterium; inflammatory response; interleukin-6 secretion; interleukin-8 secretion; negative regulation of blood pressure; negative regulation of gene expression; negative regulation of protein catabolic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; ovulation from ovarian follicle; peptidyl-cysteine S-nitrosylation; positive regulation of blood vessel diameter; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; prostaglandin secretion; regulation of cell proliferation; regulation of cytokine production involved in inflammatory response; regulation of insulin secretion; response to hypoxia; response to lipopolysaccharide; superoxide metabolic process
Research Articles on NOS2
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Product Notes
The NOS2 nos2 (Catalog #AAA2002242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nitric Oxide Synthase 2, Inducible (NOS2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nitric Oxide Synthase 2, Inducible (NOS2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NOS2 nos2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- VMASR VRATVLFATE TGKSEALARD LATLFSYAFN TKVVCMDQYK ASTLEEEQLL LVVTSTFGNG DCPSNGQTLK KSLFMLRELN HTFRYAVFGL GSSMYPQFCA FAHDIDQKLS HLGASQLAPT GEGDELSGQ. It is sometimes possible for the material contained within the vial of "Nitric Oxide Synthase 2, Inducible (NOS2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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