Rabbit NIPP1/Ppp1r8 Polyclonal Antibody | anti-Ppp1r8 antibody

Anti-NIPP1/Ppp1r8 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100864

• Gene Names: PPP1R8; ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: NIPP1/Ppp1r8; Polyclonal Antibody; Anti-NIPP1/Ppp1r8 Antibody; Rabbit Polyclonal; NIPP1/PPP1R8 Antibody; Rabbit PAb; Antigen Affinity Purified; protein phosphatase 1 regulatory subunit 8; Anti-ARD-1 Antibody; Anti-ARD1 Antibody; Anti-NIPP-1 Antibody; Anti-NIPP1 Antibody; Anti-PRO2047 Antibody; anti-Ppp1r8 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NIPP1/Ppp1r8
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-Ppp1r8 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human NIPP1/PPP1R8
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human NIPP1/PPP1R8, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PPP1R8 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:38 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of PPP1R8 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PPP1R8 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-Ppp1r8 antibody

The ubiquitously expressed nuclear protein NIPP1 (also known as PPP1R8) recruits phosphoproteins for regulated dephosphorylation by the associated protein phosphatase PP1. The deletion of NIPP1 in mice is early embryonic lethal, which has precluded functional studies in adult tissues. Nuclear Inhibitor of PP1 (NIPP1; 351 residues in mouse), encoded by Ppp1r8, is one of the major nuclear interactors of PP1 and is expressed in nearly all eukaryotic cells. The N-terminal third of NIPP1 largely consists of a well-structured ForkHead-Associated (FHA) domain, while the remainder of the protein is intrinsically disordered but becomes partially structured upon binding to PP1. The FHA domain of NIPP1 mediates the recruitment of a subset of phosphoproteins, including the pre-mRNA splicing factors SAP155 and CDC5L, protein kinase MELK and the methyltransferase EZH2, for regulated dephosphorylation by associated PP1.

References

Ferreira M, et al. (2017) The protein phosphatase 1 regulator nipp1 is essential for mammalian spermatogenesis. Sci Rep 7 (1): 13364.

NCBI and Uniprot Product Information

• NCBI GI #: 4506015
• NCBI GeneID: 5511
• NCBI Accession #: NP_002704.1
• NCBI GenBank Nucleotide #: NM_002713.3
• UniProt Accession #: Q12972; Q5TEJ2; Q5TEJ4; Q5TIF2; Q6PKF6; Q9UBH1; Q9UBZ0
• Molecular Weight: 38,479 Da
• NCBI Official Full Name: nuclear inhibitor of protein phosphatase 1 isoform gamma
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 8
• NCBI Official Symbol: PPP1R8
• NCBI Official Synonym Symbols: ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• NCBI Protein Information: nuclear inhibitor of protein phosphatase 1; RNase E; activator of RNA decay; nuclear subunit of PP-1; protein phosphatase 1 regulatory subunit 8; nuclear inhibitor of protein phosphatase-1 alpha; protein phosphatase 1, regulatory (inhibitor) subunit 8
• UniProt Protein Name: Nuclear inhibitor of protein phosphatase 1
• Protein Family: Nuclear inhibitor of protein phosphatase
• UniProt Gene Name: PPP1R8
• UniProt Synonym Gene Names: ARD1; NIPP1; NIPP-1
• UniProt Entry Name: PP1R8_HUMAN

NCBI Description

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]

Uniprot Description

NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; EC 3.1.4.-; Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit; Ribonuclease

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; ribonuclease E activity; RNA binding; protein phosphatase type 1 regulator activity; endonuclease activity; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: cell proliferation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; RNA splicing; RNA catabolic process; negative regulation of catalytic activity; mRNA processing