Standard Curve (Sample)
Human TRIM25 (E3 ubiquitin/ISG15 ligase TRIM25) ELISA Kit | TRIM25 elisa kit
Human TRIM25 (E3 ubiquitin/ISG15 ligase TRIM25) ELISA Kit
Gene Names
TRIM25; EFP; Z147; RNF147; ZNF147
Reactivity
Human
Synonyms
TRIM25 (E3 ubiquitin/ISG15 ligase TRIM25); N/A; Human TRIM25 (E3 ubiquitin/ISG15 ligase TRIM25) ELISA Kit; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase; RING-type E3 ubiquitin transferase TRIM25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147; EFP; RNF147; ZNF147; TRIM25; TRIM25 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TRIM25. No significant cross-reactivity or interference between TRIM25 and analogues was observed.
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TRIM25 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70,973 Da
NCBI Official Full Name
E3 ubiquitin/ISG15 ligase TRIM25
NCBI Official Synonym Full Names
tripartite motif containing 25
NCBI Official Symbol
TRIM25
NCBI Official Synonym Symbols
EFP; Z147; RNF147; ZNF147
NCBI Protein Information
E3 ubiquitin/ISG15 ligase TRIM25; RING finger protein 147; zinc finger protein-147; tripartite motif-containing 25; tripartite motif protein TRIM25; estrogen-responsive finger protein; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating en
UniProt Protein Name
E3 ubiquitin/ISG15 ligase TRIM25
UniProt Gene Name
TRIM25
UniProt Synonym Gene Names
EFP; RNF147; ZNF147
UniProt Entry Name
TRI25_HUMAN
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Product Notes
The Human TRIM25 trim25 (Catalog #AAA249941) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA249941 ELISA Kit recognizes Human TRIM25. It is sometimes possible for the material contained within the vial of "TRIM25 (E3 ubiquitin/ISG15 ligase TRIM25), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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