Rabbit NIPP1 Polyclonal Antibody | anti-NIPP1 antibody

NIPP1 Polyclonal Antibody

Cat. Num. AAA3011808

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NIPP1; Polyclonal Antibody; NIPP1 Polyclonal Antibody; Nuclear inhibitor of protein phosphatase 1; NIPP-1; Protein phosphatase 1 regulatory inhibitor subunit 8; Activator of RNA decay; ARD-1; PPP1R8; ARD1; anti-NIPP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NIPP1 polyclonal antibody detects endogenous levels of NIPP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 127

• Applicable Applications for anti-NIPP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human NIPP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using PPP1R8 antibody.)

Related Product Information for anti-NIPP1 antibody

NIPP1 (nuclear inhibitor of protein phosphatase 1) is a putative transcription regulator that may be involved in pre-mRNA splicing and cell proliferation. NIPP1 contains a nuclear signaling region named FHA (fork-head associated) domain. The FHA domain has been associated with protein kinases and transcription factors. The NIPP1 locus encodes for three different isoforms termed alpha, beta and gamma due to alternative splicing events. The isoforms exhibit RNA binding activity and also act as phophatase inhibitors. The gamma isoform is believed to be a magnesium dependent endoribonuclease that is responsible for cleaving RNA strands. It is mainly found in B cells and T lymphocytes. The alpha and beta isoforms are localized in the brain and kidney. Inactivation of NIPP1 is accomplished by the phosphorylation of Ser 199 or Ser 204. NIPP1 interacts with proteins CDc5L, SAP 155, MELK and EED.

NCBI and Uniprot Product Information

• NCBI GI #: 4506015
• NCBI Accession #: NP_002704.1
• NCBI GenBank Nucleotide #: NP_002704.1
• UniProt Accession #: Q12972
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: nuclear inhibitor of protein phosphatase 1 isoform gamma
• UniProt Protein Name: Nuclear inhibitor of protein phosphatase 1
• UniProt Gene Name: PPP1R8
• UniProt Synonym Gene Names: ARD1; NIPP1; NIPP-1
• UniProt Entry Name: PP1R8_HUMAN

Uniprot Description

NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; EC 3.1.4.-; Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit; Ribonuclease

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; ribonuclease E activity; RNA binding; protein phosphatase type 1 regulator activity; endonuclease activity; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: cell proliferation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; RNA splicing; RNA catabolic process; negative regulation of catalytic activity; mRNA processing