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Immunohistochemistry (IHC)
(Immunochemical staining of human GLUL in human tonsil with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Rabbit Glutamine Synthetase/GLUL Polyclonal Antibody | anti-GLUL antibody
Anti-Glutamine Synthetase/GLUL Antibody, Rabbit Polyclonal
Gene Names
Glul; GS; Glns
Reactivity
HumanPredicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
Glutamine Synthetase/GLUL; Polyclonal Antibody; Anti-Glutamine Synthetase/GLUL Antibody; Rabbit Polyclonal; GLUL Antibody; Rabbit PAb; Antigen Affinity Purified; glutamate-ammonia ligase; Anti-GLNS Antibody; Anti-GS Antibody; Anti-PIG43 Antibody; Anti-PIG59 Antibody; anti-GLUL antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Glutamine Synthetase/GLUL
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GLUL antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:2500-1:10,000
IP: 4-6uL/mg of lysate
IHC-P: 1:2500-1:10,000
IP: 4-6uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human GLUL
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human GLUL, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human GLUL in human tonsil with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human GLUL in human tonsil with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human GLUL in human glioma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human GLUL in human glioma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human GLUL in human breast with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human GLUL in human breast with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(GLUL was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-GLUL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GLUL rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDa)
Immunoprecipitation (IP)
(GLUL was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-GLUL rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GLUL rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDa)
Western Blot (WB)
(Anti-GLUL rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa)
Western Blot (WB)
(Anti-GLUL rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa)
Related Product Information for anti-GLUL antibody
Glutamate-ammonia ligase (GLUL) belongs to the glutamine synthetase family. GLUL was heterogeneously expressed in various breast cancer cells. GLUL may represent a novel target for selectively inhibiting p38 MAPK and ERK1/ERK2 signaling pathways and the proliferation potential of breast cancer cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,120 Da
NCBI Official Full Name
glutamine synthetase
NCBI Official Synonym Full Names
glutamate-ammonia ligase (glutamine synthetase)
NCBI Official Symbol
Glul
NCBI Official Synonym Symbols
GS; Glns
NCBI Protein Information
glutamine synthetase
UniProt Protein Name
Glutamine synthetase
UniProt Gene Name
Glul
UniProt Synonym Gene Names
Glns; GS
Uniprot Description
Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner ().
Research Articles on GLUL
Similar Products
Product Notes
The GLUL glul (Catalog #AAA8100607) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Glutamine Synthetase/GLUL Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamine Synthetase/GLUL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:2500-1:10,000 IP: 4-6uL/mg of lysate. Researchers should empirically determine the suitability of the GLUL glul for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamine Synthetase/GLUL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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