Rat Paired Box Gene 6 (PAX6) ELISA Kit | PAX6 elisa kit

Rat Paired Box Gene 6 (PAX6) ELISA Kit

Cat. Num. AAA2020979

• Gene Names: PAX6; AN; AN2; FVH1; MGDA; WAGR; D11S812E
• Reactivity: Rat
• Synonyms: Paired Box Gene 6 (PAX6); Rat Paired Box Gene 6 (PAX6) ELISA Kit; AN; AN2; MGDA; WAGR; Oculorhombin; Aniridia; Keratitis; Aniridia type II protein; PAX6 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Paired Box Gene 6 (PAX6).; No significant cross-reactivity or interference between Paired Box Gene 6 (PAX6) and analogues was observed.
• Sequence Length: 470

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paired Box Gene 6 (PAX6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paired Box Gene 6 (PAX6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAX6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paired Box Gene 6 (PAX6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paired Box Gene 6 (PAX6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paired Box Gene 6 (PAX6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paired Box Gene 6 (PAX6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PAX6 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119588642
• NCBI GeneID: 5080
• UniProt Accession #: P26367; Q6N006; Q99413
• Molecular Weight: – Da
• NCBI Official Full Name: paired box gene 6 (aniridia, keratitis), isoform CRA_c
• NCBI Official Synonym Full Names: paired box 6
• NCBI Official Symbol: PAX6
• NCBI Official Synonym Symbols: AN; AN2; FVH1; MGDA; WAGR; D11S812E
• NCBI Protein Information: paired box protein Pax-6
• UniProt Protein Name: Paired box protein Pax-6
• Protein Family: Paired box protein
• UniProt Gene Name: PAX6
• UniProt Synonym Gene Names: AN2
• UniProt Entry Name: PAX6_HUMAN

NCBI Description

This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

Uniprot Description

PAX6: Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells. Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Defects in PAX6 are the cause of aniridia (AN). A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time. Defects in PAX6 are a cause of Peters anomaly (PAN). Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea. Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP). Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant. Defects in PAX6 are a cause of keratitis hereditary (KERH). An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization. Defects in PAX6 are a cause of coloboma of iris choroid and retina (COI); also known as uveoretinal coloboma. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia. Defects in PAX6 are a cause of coloboma of optic nerve (COLON). Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH); also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary. Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD); also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation. Belongs to the paired homeobox family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; AT DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; ubiquitin-protein ligase activity; chromatin binding; transcription factor binding; transcription factor activity; protein kinase binding

Biological Process: central nervous system development; positive regulation of transcription, DNA-dependent; protein ubiquitination; cell fate determination; glucose homeostasis; positive regulation of epithelial cell differentiation; cerebellum development; neural crest cell migration; positive regulation of neuroblast proliferation; positive regulation of cell fate specification; neuron fate commitment; oligodendrocyte cell fate specification; interkinetic nuclear migration; keratinocyte differentiation; forebrain-midbrain boundary formation; eye photoreceptor cell development; negative regulation of neuron differentiation; organ morphogenesis; response to ethanol; cellular response to insulin stimulus; retina development in camera-type eye; rhombomere morphogenesis; regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment; forebrain anterior/posterior pattern formation; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; lens development in camera-type eye; axon guidance; regulation of asymmetric cell division; neuron migration; forebrain dorsal/ventral pattern formation; dorsal/ventral axis specification; negative regulation of transcription from RNA polymerase II promoter; cerebral cortex regionalization; negative regulation of neurogenesis; establishment of mitotic spindle orientation; negative regulation of protein amino acid phosphorylation; astrocyte differentiation; visual perception; salivary gland morphogenesis; response to wounding; lacrimal gland development; regulation of timing of cell differentiation; negative regulation of epithelial cell proliferation; blood vessel development; smoothened signaling pathway; olfactory bulb development; eye development; commitment of a neuronal cell to a specific type of neuron in the forebrain; protein localization in organelle; pituitary gland development; regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification; regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification; embryonic camera-type eye morphogenesis

Disease: Wilms Tumor, Aniridia, Genitourinary Anomalies, And Mental Retardation Syndrome; Peters Anomaly; Coloboma Of Optic Nerve; Keratitis, Hereditary; Aniridia, Cerebellar Ataxia, And Mental Retardation; Foveal Hypoplasia 1; Aniridia; Optic Nerve Hypoplasia, Bilateral