Human Disintegrin and metalloproteinase domain-containing protein 29 ELISA Kit | ADAM29 elisa kit

Human Disintegrin and metalloproteinase domain-containing protein 29 ELISA Kit

Cat. Num. AAA763102

• Gene Names: ADAM29; CT73; svph1
• Reactivity: Human
• Synonyms: Disintegrin and metalloproteinase domain-containing protein 29; Human Disintegrin and metalloproteinase domain-containing protein 29 ELISA Kit; ADAM29/Cancer/testis antigen 73 (CT73)/; ADAM29 elisa kit

• Reactivity: Human
• Sequence Length: 820

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ADAM29 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human ADAM29 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ADAM29 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAM29 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ADAM29 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 73765552
• NCBI GeneID: 11086
• NCBI Accession #: NP_055084.3
• NCBI GenBank Nucleotide #: NM_014269.4
• Molecular Weight: 89,460 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 29 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 29
• NCBI Official Symbol: ADAM29
• NCBI Official Synonym Symbols: CT73; svph1
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 29
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 29
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM29
• UniProt Synonym Gene Names: ADAM 29; CT73
• UniProt Entry Name: ADA29_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is highly expressed in testis and may be involved in human spermatogenesis. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM29: a member of the ADAM (a disintegrin and metalloprotease domain) family of Cancer-Testis Antigen (CTAs). CTAs may play roles in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovarian cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins. May be involved in spermatogenesis and fertilization. Seems to be a non catalytic metalloprotease-like protein. Has been found to be frequently mutated in melanoma. Mutations may play a role in melanoma progression and metastasis. Expressed in leukemias at the mRNA level. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Protease; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 4q34

Cellular Component: integral to plasma membrane

Molecular Function: metalloendopeptidase activity; metallopeptidase activity; zinc ion binding

Biological Process: proteolysis; spermatogenesis