Rabbit anti-Human, Mouse ZC3HC1 Polyclonal Antibody | anti-ZC3HC1 antibody

ZC3HC1(Phospho-S354) Polyclonal Antibody

Cat. Num. AAA3007951

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ZC3HC1; Polyclonal Antibody; ZC3HC1(Phospho-S354) Polyclonal Antibody; Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1; NIPA; HSPC216; anti-ZC3HC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ZC3HC1(Phospho-S354) polyclonal antibody detects endogenous levels of ZC3HC1 protein only when phosphorylated at Ser354.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
• Sequence Length: 502

• Applicable Applications for anti-ZC3HC1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human ZC3HC1 around the phosphorylation site of Serine 354.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ZC3HC1 (Phospho-S354) polyclonal antibody at 1:500 dilutionLane1:K562 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:Jurkat whole cell lysate (40ug))

Related Product Information for anti-ZC3HC1 antibody

NIPA (nuclear interaction partner of ALK) is an F-box-containing protein that is an essential component of the SCF-type E3 ligase (SCFNIPA) complex, a complex that controls the completion of S-phase and mitotic entry. This control is mediated by the ubiquitination and subsequent degradation of cell cycle regulatory proteins, whose oscillation of protein levels is required for proper cell cycle progression. Expression levels of NIPA are low in G0/G1 phases and upregulated in S and G2/M phases. The SCFNIPA complex targets nuclear cyclin B1 for ubiquitination in interphase, whereas phosphorylation of NIPA in late G2 phase and mitosis inactivates the complex to allow for accumulation of cyclin B1. NIPA may have an anti-apoptotic role in NPM-ALK-mediated signaling events.

NCBI and Uniprot Product Information

• NCBI GI #: 66348045
• NCBI Accession #: NP_057562.3
• NCBI GenBank Nucleotide #: NP_057562.3
• UniProt Accession #: Q86WB0
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: nuclear-interacting partner of ALK isoform 1
• UniProt Protein Name: Nuclear-interacting partner of ALK
• Protein Family: NIPA-like protein
• UniProt Gene Name: ZC3HC1
• UniProt Synonym Gene Names: NIPA; hNIPA
• UniProt Entry Name: NIPA_HUMAN

Uniprot Description

NIPA: a nuclear protein that interacts with anaplastic lymphoma kinase (ALK). An essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1. Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting cyclin B1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of cyclin B1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. Interacts with the NPM-ALK fusion protein in a tyrosine phosphorylation-dependent manner. Three splice-variant isoforms have been described.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 7q32.2

Cellular Component: nuclear membrane; nucleus

Molecular Function: protein binding; zinc ion binding; protein kinase binding

Biological Process: mitosis; cell division; protein ubiquitination