Rabbit Nicotinic Acetylcholine Receptor (nAChR) Polyclonal Antibody | anti-Chrnb4 antibody

Nicotinic Acetylcholine Receptor (nAChR) b4

Cat. Num. AAA500064

• Gene Names: Chrnb4; Acrb4; Acrb-4
• Reactivity: Predicted: Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified from Pooled Serum
• Synonyms: Nicotinic Acetylcholine Receptor (nAChR); Polyclonal Antibody; Nicotinic Acetylcholine Receptor (nAChR) b4; anti-Chrnb4 antibody

• Host: Rabbit
• Reactivity: Predicted: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified from Pooled Serum
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 495

• Applicable Applications for anti-Chrnb4 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000
• Immunogen: Fusion protein from the cytoplasmic loop of the beta 4 subunit of rat nAChR; Immunogen Species
• Purification Note: The antibody is prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
• Preparation and Storage: Store at -20 degree C

Related Product Information for anti-Chrnb4 antibody

Nicotinic acetylcholine receptors (nAChRs) are ionotropic, cholinergic receptors that are divided into 2 types; muscle type and neuronal type. Neuronal nAChRs are pentameric ion channels consisting of 5 identical (homopentamers) or different (heteropentamers) subunits. Heteropentameric neuronal nAChRs mediate fast synaptic transmission in the autonomic nervous system. The predominant hetero-oligomeric nAChR in the CNS contain the subunits alpha4beta2, whereas alpha3beta4 prevail in the PNS. However, the expression of these subunits varies not only by region but also during development (Scholze et al 2011). In the brain, beta2-containing receptors greatly outnumber receptors that contain beta4 (McGehee & Role, 1995; Albuquerque et al., 2009), and in most brain regions, targeted deletion of the beta2 subunit virtually abolishes [3H]-epibatidine binding and receptor autoradiography (Zoli et al., 1998) due to the absence of a beta subunit required to form functional nAChRs (Champtiaux & Changeux, 2004).

Product Categories/Family for anti-Chrnb4 antibody

Neurotransmission

NCBI and Uniprot Product Information

• NCBI GI #: 33563301
• NCBI GeneID: 108015
• NCBI Accession #: NP_683746.1
• NCBI GenBank Nucleotide #: NM_148944.4
• UniProt Accession #: Q8R493; Q8VI06
• Molecular Weight: ~52 kDa
• NCBI Official Full Name: neuronal acetylcholine receptor subunit beta-4
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, beta polypeptide 4
• NCBI Official Symbol: Chrnb4
• NCBI Official Synonym Symbols: Acrb4; Acrb-4
• NCBI Protein Information: neuronal acetylcholine receptor subunit beta-4
• UniProt Protein Name: Neuronal acetylcholine receptor subunit beta-4
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: Chrnb4
• UniProt Synonym Gene Names: Acrb4

Uniprot Description

nAChRB4: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 4/CHRNB4 sub-subfamily.

Protein type: Channel, cation; Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Receptor, misc.

Chromosomal Location of Human Ortholog: 9|9 B

Cellular Component: membrane; neuron projection; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane

Molecular Function: acetylcholine binding; acetylcholine receptor activity; acetylcholine-gated cation channel activity; drug binding; protein heterodimerization activity

Biological Process: behavioral response to nicotine; locomotory behavior; positive regulation of transmission of nerve impulse; protein heterooligomerization; regulation of action potential; regulation of membrane potential; regulation of smooth muscle contraction; response to nicotine; signal transduction; smooth muscle contraction; synaptic transmission involved in micturition; synaptic transmission, cholinergic