Rabbit anti-Human Nicastrin Polyclonal Antibody | anti-NCSTN antibody

Nicastrin

Cat. Num. AAA620490

• Gene Names: NCSTN; APH2; KIAA0253; RP11-517F10.1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Nicastrin; Polyclonal Antibody; Anti -Nicastrin; anti-NCSTN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes Human Nicastrin (NCSTN). Species Crossreactivity: Human, Mouse, Rat.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-NCSTN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry and Western Blot.; Dilution: Immunohistochemistry: 10ug/ml
• Immunogen: Synthetic peptide, KLH-conjugated. Epitope: 17 amino acid peptide from near the carboxy terminus of human Nicastrin
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(NCSTN / Nicastrin (10ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Product Categories/Family for anti-NCSTN antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 9992878
• NCBI GeneID: 23385
• UniProt Accession #: Q5T208; Q5T209; Q92542
• Molecular Weight: 78,411 Da
• NCBI Official Full Name: nicastrin
• NCBI Official Synonym Full Names: nicastrin
• NCBI Official Symbol: NCSTN
• NCBI Official Synonym Symbols: APH2; KIAA0253; RP11-517F10.1
• NCBI Protein Information: nicastrin; ATAG1874; OTTHUMP00000031839; OTTHUMP00000031840; anterior pharynx-defective 2
• UniProt Protein Name: Nicastrin
• Protein Family: Nicastrin
• UniProt Gene Name: NCSTN
• UniProt Entry Name: Q5T208_HUMAN

NCBI Description

This gene encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq]

Uniprot Description

nicastrin: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP). Constitutively expressed in neural cells. Widely expressed. Belongs to the nicastrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q23

Cellular Component: Golgi apparatus; focal adhesion; membrane; integral to plasma membrane; lysosomal membrane; endoplasmic reticulum; melanosome; plasma membrane; integral to membrane

Molecular Function: protein binding; endopeptidase activity

Biological Process: positive regulation of catalytic activity; axon guidance; extracellular matrix organization and biogenesis; Notch signaling pathway; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; myeloid cell homeostasis; membrane protein ectodomain proteolysis; Notch receptor processing; proteolysis; T cell proliferation; extracellular matrix disassembly; epithelial cell proliferation; amyloid precursor protein catabolic process; beta-amyloid metabolic process; ephrin receptor signaling pathway; protein processing

Disease: Acne Inversa, Familial, 1