Rabbit NHERF2 Polyclonal Antibody | anti-NHERF2 antibody

NHERF2 antibody

Cat. Num. AAA835516

• Gene Names: SLC9A3R2; SIP1; OCTS2; SIP-1; TKA-1; E3KARP; NHERF2; NHE3RF2; NHERF-2
• Applications: Western Blot
• Purity: NHERF2 antibody was purified by Protein A chromatography
• Synonyms: NHERF2; Polyclonal Antibody; NHERF2 antibody; Polyclonal NHERF2; Anti-NHERF2; SIP-1; solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulator 2; TKA-1; NHERF-2; Na(+)/H(+) exchange regulatory cofactor NHE-RF2; E3KARP; sodium/hydrogen exchanger 3 kinase A regulatory protein; MGC104639; NHERF 2; SLC9A3R2; tyrosine kinase activator protein 1; NHE3RF2; solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 2; SIP1; OCTS2; NHE3 regulatory factor 2; NHE3 kinase A regulatory protein E3KARP; SRY-interacting protein 1; solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulatory factor 2; solute carrier family 9 isoform A3 regulatory factor 2; anti-NHERF2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NHERF2 antibody was purified by Protein A chromatography
• Form/Format: Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
• Concentration: 5.91 mg/ml (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-NHERF2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:3000
• Biological Significance: NHE3 is a sodium/hydrogen exchanger in the brush border membrane of the proximal tubule, small intestine, and colon that plays a major role in transepithelial sodium absorption. SLC9A3R2, as well as SLC9A3R1 and protein kinase A phosphorylation, may play a role in NHE3 regulation.
• Cross-Reactivity: Human, Mouse
• Immunogen: NHERF2 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 82 and 307 of NHERF-2
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 50 ug of whole cell lysate (A: mouse liver) using a 15 % SDS PAGE gel and NHERF2 antibody at a dilution of 1:1000)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A:NT2D1) using a 10 % SDS PAGE gel and NHERF2 antibody at a dilution of 1:1000)

Related Product Information for anti-NHERF2 antibody

Affinity purified Rabbit polyclonal NHERF2 antibody

Product Categories/Family for anti-NHERF2 antibody

Cell Biology; Purified Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 355330272
• NCBI GeneID: 9351
• NCBI Accession #: NP_001239005.1
• NCBI GenBank Nucleotide #: NM_001252076.1
• UniProt Accession #: Q15599; O00272; O00556; Q3KQY7; D3DU84; D3DU85; H3BSV6
• Molecular Weight: 36 kDa
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF2 isoform e
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2
• NCBI Official Symbol: SLC9A3R2
• NCBI Official Synonym Symbols: SIP1; OCTS2; SIP-1; TKA-1; E3KARP; NHERF2; NHE3RF2; NHERF-2
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF2
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF2
• UniProt Gene Name: SLC9A3R2
• UniProt Synonym Gene Names: NHERF2; NHERF-2; SIP-1; TKA-1
• UniProt Entry Name: NHRF2_HUMAN

NCBI Description

This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

NHERF-2: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus. Homodimer, and heterodimer with SLC9A3R1. Binds PDZK1. Found in a complex with EZR, PODXL and SLC9A3R2. Interacts (via the PDZ domains) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is detected in glomerular epithelium cells. Binds ADRB2, SLC9A3, P2RY1, P2YR2, SRY, RDX and LPAR2. Interacts with MCC and PODXL. Interacts with SGK1 and KCNJ1/ROMK1. Widely expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: focal adhesion; apical plasma membrane; cytoplasm; plasma membrane; endomembrane system; nucleus

Molecular Function: protein C-terminus binding; protein binding; beta-catenin binding; phosphatase binding; receptor binding

Biological Process: protein complex assembly; negative regulation of phosphoinositide 3-kinase cascade