Thrombopoietin Recombinant Protein | THPO recombinant protein

Thrombopoietin Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120238

• Gene Names: THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Thrombopoietin; Thrombopoietin Protein; Human; Recombinant (His Tag); Human Thrombopoietin/THPO Protein (His Tag); MGDF Protein; MKCSF Protein; ML Protein; MPLLG Protein; THCYT1 Protein; TPO Protein; thrombopoietin; THPO recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly353

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ser 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human THPO (NP_000451.1)(Met1-Gly353) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for THPO recombinant protein

Background: Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action.

Product Categories/Family for THPO recombinant protein

Serine Proteases and Regulators

References

Ryu T,et al.(2003) Thrombopoietin-producing hepatocellular carcinoma. Intern Med. 42(8): 730-4.Higashihara M,et al. (2003) Thrombopoietin-producing tumor. Intern Med. 42(8): 632-3

NCBI and Uniprot Product Information

• NCBI GI #: 4507493
• NCBI GeneID: 7066
• NCBI Accession #: NP_000451.1
• NCBI GenBank Nucleotide #: NM_000460.3
• UniProt Accession #: P40225; Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8
• Molecular Weight: 33,604 Da
• NCBI Official Full Name: thrombopoietin isoform 1
• NCBI Official Synonym Full Names: thrombopoietin
• NCBI Official Symbol: THPO
• NCBI Official Synonym Symbols: ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
• NCBI Protein Information: thrombopoietin
• UniProt Protein Name: Thrombopoietin
• Protein Family: Thrombopoietin
• UniProt Gene Name: THPO
• UniProt Synonym Gene Names: MGDF; ML; MGDF
• UniProt Entry Name: TPO_HUMAN

Uniprot Description

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function: growth factor activity; hormone activity; cytokine activity

Biological Process: positive regulation of protein kinase B signaling cascade; cell proliferation; platelet activation; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation

Disease: Thrombocythemia 1