Western Blot (WB)
(Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400). Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400) at 1:1000.)
Rabbit anti-Rat, Mouse NHE3 Polyclonal Antibody | anti-Slc9a3 antibody
NHE3 Antibody
Western Blot (WB)
(Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400). Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400) at 1:1000.)
Western Blot (WB)
(Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400). Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400) at 1:1000.)
Scientific Background: Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
NCBI and Uniprot Product Information
NCBI Description
displays Na+/H+ exchanger transport activity; mediates sodium ion transport across the renal proximal tubule [RGD, Feb 2006]
Uniprot Description
NHE3: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. Binds SLC9A3R1 and SLC9A3R2. Interacts with SHANK2. Interacts with PDZD3 and interactions decrease in response to elevated calcium ion levels. Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, SLC family
Cellular Component: brush border membrane; apical plasma membrane; plasma membrane; integral to membrane; brush border
Molecular Function: protein binding; sodium:hydrogen antiporter activity; PDZ domain binding
Biological Process: circadian rhythm; receptor-mediated endocytosis; sodium ion transport; response to glucocorticoid stimulus; regulation of pH; regulation of sodium ion transport
Research Articles on Slc9a3
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Product Notes
The Slc9a3 slc9a3 (Catalog #AAA806469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NHE3 Antibody reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NHE3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). 1:1000 (WB). Researchers should empirically determine the suitability of the Slc9a3 slc9a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NHE3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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