Rat Sodium/Hydrogen Exchanger 3 (NHE3) ELISA Kit | NHE3 elisa kit

Rat Sodium/Hydrogen Exchanger 3 (NHE3) ELISA Kit

Cat. Num. AAA2705483

• Gene Names: SLC9A3; NHE3; DIAR8; NHE-3
• Reactivity: Rat
• Synonyms: Sodium/Hydrogen Exchanger 3 (NHE3); Rat Sodium/Hydrogen Exchanger 3 (NHE3) ELISA Kit; SLC9A3; Solute Carrier Family 9 Member 3; Sodium-hydrogen antiporter 3; Na(+)/H(+) exchanger 3; NHE3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sodium/Hydrogen Exchanger 3 (NHE3).; No significant cross-reactivity or interference between Sodium/Hydrogen Exchanger 3 (NHE3) and analogues was observed.
• Sequence Length: 825

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.126ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sodium/Hydrogen Exchanger 3 (NHE3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sodium/Hydrogen Exchanger 3 (NHE3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NHE3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Hydrogen Exchanger 3 (NHE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Hydrogen Exchanger 3 (NHE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Hydrogen Exchanger 3 (NHE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Hydrogen Exchanger 3 (NHE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 194239733
• NCBI GeneID: 6550
• NCBI Accession #: NP_004165.2
• NCBI GenBank Nucleotide #: NM_004174.3
• UniProt Accession #: P48764; Q3MIW3; B7ZKR2; E9PF67
• Molecular Weight: 91,904 Da
• NCBI Official Full Name: sodium/hydrogen exchanger 3 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 9 member A3
• NCBI Official Symbol: SLC9A3
• NCBI Official Synonym Symbols: NHE3; DIAR8; NHE-3
• NCBI Protein Information: sodium/hydrogen exchanger 3
• UniProt Protein Name: Sodium/hydrogen exchanger 3
• UniProt Gene Name: SLC9A3
• UniProt Synonym Gene Names: NHE-3

NCBI Description

The protein encoded by this gene is an epithelial brush border Na/H exchanger that uses an inward sodium ion gradient to expel acids from the cell. Defects in this gene are a cause of congenital secretory sodium diarrhea. Pseudogenes of this gene exist on chromosomes 10 and 22. [provided by RefSeq, Mar 2016]

Uniprot Description

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient (PubMed:26358773). Plays an important role in signal transduction.