Western Blot (WB)
(Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401). Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401) at 1:1000.)
Rabbit NKCC2 Polyclonal Antibody | anti-Slc12a1 antibody
NKCC2 Antibody: ATTO 390
Western Blot (WB)
(Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401). Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401) at 1:1000.)
Western Blot (WB)
(Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401). Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401) at 1:1000.)
Scientific Background: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
NCBI and Uniprot Product Information
NCBI Description
has bumetamide-sensitive Na(+)-(K+)-Cl- symporter activity; may play a role in sodium and chloride transport in the kidney [RGD, Feb 2006]
Uniprot Description
NKCC2: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Kidney specific. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter, SLC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass
Cellular Component: cell surface; apical plasma membrane; integral to membrane; plasma membrane
Molecular Function: sodium:potassium:chloride symporter activity; cation:chloride symporter activity
Biological Process: diterpenoid metabolic process; protein insertion into membrane from inner side; sodium ion transport; chloride transport; kidney development; excretion; potassium ion transport; chemical homeostasis
Research Articles on Slc12a1
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Product Notes
The Slc12a1 slc12a1 (Catalog #AAA804930) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NKCC2 Antibody: ATTO 390 reacts with Human (weak), Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NKCC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoblot (IB). 1:1000 (WB). Researchers should empirically determine the suitability of the Slc12a1 slc12a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKCC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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