Rabbit NHE-7 Polyclonal Antibody | anti-NHE-7 antibody

NHE-7 (D566) Polyclonal Antibody

Cat. Num. AAA3003700

• Gene Names: SLC9A7; NHE7; NHE-7; MRX108; SLC9A6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NHE-7; Polyclonal Antibody; NHE-7 (D566) Polyclonal Antibody; Sodium/hydrogen exchanger 7; Na(+)/H(+) exchanger 7; Solute carrier family 9 member 7; SLC9A7; NHE7; anti-NHE-7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NHE-7 (D566) polyclonal antibody detects endogenous levels of NHE-7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 725

• Applicable Applications for anti-NHE-7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 530-582 of Human NHE-7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NHE-7 (D566) pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug))

Related Product Information for anti-NHE-7 antibody

Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. NHE7 (human 725 aa, chromosome Xp11.4) is ubiquitously expressed, and predominantly localizes to the trans-golgi network. NHE7 mediates the influx of Na+ or K+ in exchange for H+. It is ~70% related to NHE6 but relatively less (~25%) homologous with other NHEs.

NCBI and Uniprot Product Information

• NCBI GI #: 14211919
• NCBI GeneID: 84679
• NCBI Accession #: NP_115980.1
• NCBI GenBank Nucleotide #: NP_115980.1
• UniProt Accession #: Q96T83
• Molecular Weight: ~ 80kDa
• NCBI Official Full Name: sodium/hydrogen exchanger 7 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 9 member A7
• NCBI Official Symbol: SLC9A7
• NCBI Official Synonym Symbols: NHE7; NHE-7; MRX108; SLC9A6
• NCBI Protein Information: sodium/hydrogen exchanger 7
• UniProt Protein Name: Sodium/hydrogen exchanger 7
• UniProt Gene Name: SLC9A7
• UniProt Synonym Gene Names: NHE7; NHE-7
• UniProt Entry Name: SL9A7_HUMAN

NCBI Description

This gene encodes a sodium and potassium/ proton antiporter that is a member of the solute carrier family 9 protein family. The encoded protein is primarily localized to the trans-Golgi network and is involved in maintaining pH homeostasis in organelles along the secretory and endocytic pathways. This protein may enhance cell growth of certain breast tumors. This gene is part of a gene cluster on chromosome Xp11.23. A pseudogene of this gene is found on chromosome 12. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]

Uniprot Description

NHE7: Mediates electroneutral exchange of protons for Na(+) and K(+) across endomembranes. May contribute to Golgi volume and cation homeostasis. Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

Protein type: Transporter, SLC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xp11.3|Xp11.3

Cellular Component: Golgi membrane; recycling endosome membrane; integral to membrane; trans-Golgi network

Molecular Function: protein binding; protein homodimerization activity; potassium:hydrogen antiporter activity; sodium:hydrogen antiporter activity

Biological Process: regulation of pH; ion transport; transmembrane transport