Rabbit anti-Human SLC9A8 Polyclonal Antibody | anti-SLC9A8 antibody

SLC9A8 Polyclonal Antibody

Cat. Num. AAA3007623

• Gene Names: SLC9A8; NHE8; NHE-8
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SLC9A8; Polyclonal Antibody; SLC9A8 Polyclonal Antibody; Sodium/hydrogen exchanger 8; Na(+)/H(+) exchanger 8; NHE-8; Solute carrier family 9 member 8; KIAA0939; NHE8; anti-SLC9A8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SLC9A8 polyclonal antibody detects endogenous levels of SLC9A8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 597

• Applicable Applications for anti-SLC9A8 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 450-500 of Human SLC9A8.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SLC9A8 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug))

Related Product Information for anti-SLC9A8 antibody

Na+/H+ exchangers 1-8, also designated Na+/H+ antiporters or NHE-1-8, are integral membrane proteins expressed in most mammalian tissues where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, NHE isoforms 2-8 have distinct tissue and cell type dependent expression and inhibitory characteristics. NHE-8 is a 575 amino acid protein that localizes apically in intestinal epithelial cells. Expression of NHE-8 is higher in young mammals than adults. NHE-8 gene and protein expression are highly regulated during ontogeny; this protein may play an important role in intestinal Na+ absorption during early mammalian life.

NCBI and Uniprot Product Information

• NCBI GI #: 386781486
• NCBI GeneID: 23315
• NCBI Accession #: NP_001247420.1
• NCBI GenBank Nucleotide #: NP_001247420.1
• UniProt Accession #: Q9Y2E8
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: sodium/hydrogen exchanger 8 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 9 member A8
• NCBI Official Symbol: SLC9A8
• NCBI Official Synonym Symbols: NHE8; NHE-8
• NCBI Protein Information: sodium/hydrogen exchanger 8
• UniProt Protein Name: Sodium/hydrogen exchanger 8
• UniProt Gene Name: SLC9A8
• UniProt Synonym Gene Names: KIAA0939; NHE8; NHE-8
• UniProt Entry Name: SL9A8_HUMAN

NCBI Description

Sodium-hydrogen exchangers (NHEs), such as SLC9A8, are integral transmembrane proteins that exchange extracellular Na+ for intracellular H+. NHEs have multiple functions, including intracellular pH homeostasis, cell volume regulation, and electroneutral NaCl absorption in epithelia (Xu et al., 2008 [PubMed 18209477]).[supplied by OMIM, Apr 2009]

Uniprot Description

NHE8: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, SLC family

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: potassium:hydrogen antiporter activity; sodium:hydrogen antiporter activity

Biological Process: regulation of intracellular pH; ion transport; transmembrane transport