Rabbit anti-Human b2-Microglobulin Polyclonal Antibody | anti-HLA-G antibody

b2-Microglobulin (B2M)

Cat. Num. AAA613633

• Gene Names: HLA-G; MHC-G
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Highly Purified; Purified by Ion Exchange chromatography.
• Synonyms: b2-Microglobulin; Polyclonal Antibody; b2-Microglobulin (B2M); Anti -b2-Microglobulin (B2M); anti-HLA-G antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human Beta 2-microglobulin (urine).
• Purity/Purification: Highly Purified; Purified by Ion Exchange chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

• Applicable Applications for anti-HLA-G antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: ELISA: 1:2000-1:10,000; Western Blot: 1:100-1:5000; Immunohistochemistry (Paraffin): 5ug/ml; Immunoprecipitation: 1:2000-1:10,000
• Immunogen: Purified human B2M from urine.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Thymus: Formalin-Fixed Paraffin-Embedded (FFPE) using MBS613633.)

Related Product Information for anti-HLA-G antibody

Beta 2-microglobulin is the low molecular weight component of HLA antigens and as such is involved in the presentation of endogenous antigens, activation of T lymphocytes and functional emergence of natural killer cells. It is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning and other conditions leading renal tubular dysfunction. ESTs have been isolated from libraries generated using virtually every tissue in the body.

Product Categories/Family for anti-HLA-G antibody

Antibodies; Abs to Serum Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 188468
• NCBI GeneID: 3135
• UniProt Accession #: O02923; O02924; O02925; O02926; O02927; O02928; O02929; O19588; O19658; O19659
• Molecular Weight: 31,549 Da
• NCBI Official Full Name: b2 microglobulin
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, G
• NCBI Official Symbol: HLA-G
• NCBI Official Synonym Symbols: MHC-G
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; OTTHUMP00000029218; OTTHUMP00000029220; OTTHUMP00000033527; OTTHUMP00000159507; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G
• UniProt Protein Name: HLA-G protein
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-G
• UniProt Entry Name: O02923_HUMAN

NCBI Description

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq]

Uniprot Description

Sequence similarities: Belongs to the MHC class I family. RuleBase RU004439