Mouse Endonuclease 8-like 3 ELISA Kit | Neil3 elisa kit

Mouse Endonuclease 8-like 3 ELISA Kit

Cat. Num. AAA760985

• Gene Names: NEIL3; FGP2; FPG2; NEI3; ZGRF3; hFPG2; hNEI3
• Reactivity: Mouse
• Synonyms: Endonuclease 8-like 3; Mouse Endonuclease 8-like 3 ELISA Kit; Neil3/Endonuclease 8-like 3/Nei-like protein 3/Endonuclease VIII-like 3/DNA glycosylase FPG2/DNA glycosylase/AP lyase Neil3; Neil3 elisa kit

• Reactivity: Mouse
• Sequence Length: 605

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Neil3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Neil3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Neil3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Neil3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Neil3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157388969
• NCBI GeneID: 55247
• NCBI Accession #: NP_060718.2
• NCBI GenBank Nucleotide #: NM_018248.2
• Molecular Weight: 67,769 Da
• NCBI Official Full Name: endonuclease 8-like 3
• NCBI Official Synonym Full Names: nei like DNA glycosylase 3
• NCBI Official Symbol: NEIL3
• NCBI Official Synonym Symbols: FGP2; FPG2; NEI3; ZGRF3; hFPG2; hNEI3
• NCBI Protein Information: endonuclease 8-like 3
• UniProt Protein Name: Endonuclease 8-like 3
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL3
• UniProt Entry Name: NEIL3_HUMAN

NCBI Description

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]

Uniprot Description

NEIL3: Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single- stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected. Belongs to the FPG family.

Protein type: DNA-binding; EC 4.2.99.18

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: bubble DNA binding; damaged DNA binding; DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; double-stranded DNA binding; single-stranded DNA binding; zinc ion binding

Biological Process: base-excision repair; nucleotide-excision repair