Rabbit anti-Mouse CD281 Polyclonal Antibody | anti-CD281 antibody

RABBIT ANTI HUMAN CD281 (N-TERMINAL)

Cat. Num. AAA221470

• Gene Names: TLR1; TIL; CD281; rsc786; TIL. LPRS5
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Synonyms: CD281; Polyclonal Antibody; RABBIT ANTI HUMAN CD281 (N-TERMINAL); anti-CD281 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 786

• Applicable Applications for anti-CD281 antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blot: Rabbit anti Human CD281 detects a band of approximately 90kDa in mouse spleen lysates.; Immunohistology - Frozen: Maximum Dilution: 10ug/ml; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 4ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Histology Positive Control Tissue: Mouse spleen.; Buffer Solution
• Antiserum Preparation: Antisera to CD281 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of mouse spleen lysate probed with Rabbit anti Human CD281 (MBS221470) at 1(A), 2(B) and 4(C) ug/ml)

Testing Data #2

(Immunohistochemical staining of mouse spleen with Rabbit anti Human CD281(MBS221470))

Related Product Information for anti-CD281 antibody

Rabbit anti Human CD281 antibody detects an epitope within the N-terminal (NT) region of CD281, also known as toll like receptor 1 (TLR1). TLRs recognize pathogen associated molecular patterns (PAMPs) that are expressed on infectious agents, and serve as an important link between the innate and adaptive immune response through the production of cytokines. TLR1 is co-expressed with TLR2 on monocytes and dendritic cells where they form heterodimers that can recognize peptidoglycan and bacterial lipopeptides, and in particular triacylated lipoproteins. TLR1 can also regulate other TLR responses; TLR1 can associate with TLR4 and inhibit its signalling in endothelial cells, and can associate with TLR2 to inhibit the TLR2/TLR6 mediated response to phenol-soluble modulin.

NCBI and Uniprot Product Information

• NCBI GI #: 41350337
• NCBI GeneID: 7096
• NCBI Accession #: NP_003254.2
• NCBI GenBank Nucleotide #: NM_003263.3
• UniProt Accession #: Q15399; O15452; Q32MK3; Q32MK4; Q9UG90; D1CS39; D1CS41
• Molecular Weight: 90,291 Da
• NCBI Official Full Name: toll-like receptor 1
• NCBI Official Synonym Full Names: toll-like receptor 1
• NCBI Official Symbol: TLR1
• NCBI Official Synonym Symbols: TIL; CD281; rsc786; TIL. LPRS5
• NCBI Protein Information: toll-like receptor 1; toll/interleukin-1 receptor-like protein
• UniProt Protein Name: Toll-like receptor 1
• UniProt Gene Name: TLR1
• UniProt Synonym Gene Names: KIAA0012; TIL
• UniProt Entry Name: TLR1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR1: Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Binds MYD88 (via TIR domain). Interacts with CNPY3. Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2. Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: phagocytic vesicle membrane; membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; receptor activity

Biological Process: MyD88-dependent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; macrophage activation; regulation of cytokine secretion; positive regulation of interleukin-6 biosynthetic process; innate immune response; immune response; detection of triacylated bacterial lipoprotein; signal transduction; inflammatory response; toll-like receptor 1 signaling pathway

Disease: Leprosy, Susceptibility To, 5