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Testing Data (Gel: 6%SDS-PAGE Lysates (from left to right): Raji, hepG2 and hela cell Amount of lysate: 40ug per lane Primary antibody: 1/250 dilution Secondary antibody dilution: 1/8000 Exposure time: 20 seconds)

Rabbit anti-Human NFE2L1 Polyclonal Antibody | anti-NFE2L1 antibody

NFE2L1 Antibody

Gene Names
NFE2L1; NRF1; TCF11; LCR-F1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
NFE2L1; Polyclonal Antibody; NFE2L1 Antibody; NRF1; TCF11; LCR-F1; anti-NFE2L1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total NFE2L1 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
772
Applicable Applications for anti-NFE2L1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Immunogen Type
Recombinant Protein
Immunogen Description
Fusion protein corresponding to residues near the C terminal of human nuclear factor, erythroid 2-like 1
Target Name
NFE2L1
Preparation and Storage
Store at -20 degree C

Testing Data

(Gel: 6%SDS-PAGE Lysates (from left to right): Raji, hepG2 and hela cell Amount of lysate: 40ug per lane Primary antibody: 1/250 dilution Secondary antibody dilution: 1/8000 Exposure time: 20 seconds)

Testing Data (Gel: 6%SDS-PAGE Lysates (from left to right): Raji, hepG2 and hela cell Amount of lysate: 40ug per lane Primary antibody: 1/250 dilution Secondary antibody dilution: 1/8000 Exposure time: 20 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)
Related Product Information for anti-NFE2L1 antibody
This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene, NFE2L1, and for "nuclear respiratory factor 1" which has an official symbol of NRF1.
Product Categories/Family for anti-NFE2L1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81,499 Da
NCBI Official Full Name
nuclear factor erythroid 2-related factor 1
NCBI Official Synonym Full Names
nuclear factor, erythroid 2-like 1
NCBI Official Symbol
NFE2L1
NCBI Official Synonym Symbols
NRF1; TCF11; LCR-F1
NCBI Protein Information
nuclear factor erythroid 2-related factor 1
UniProt Protein Name
Nuclear factor erythroid 2-related factor 1
UniProt Gene Name
NFE2L1
UniProt Synonym Gene Names
HBZ17; NRF1; TCF11; NF-E2-related factor 1; NFE2-related factor 1; TCF-11
UniProt Entry Name
NF2L1_HUMAN

NCBI Description

This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene, NFE2L1, and for "nuclear respiratory factor 1" which has an official symbol of NRF1. [provided by RefSeq, Jul 2008]

Uniprot Description

NFE2L1: Activates erythroid-specific, globin gene expression. Interacts with KEAP1. Belongs to the bZIP family. CNC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: endoplasmic reticulum membrane; integral to membrane; nucleus

Molecular Function: transcription cofactor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; anatomical structure morphogenesis; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; heme biosynthetic process

Research Articles on NFE2L1

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Product Notes

The NFE2L1 nfe2l1 (Catalog #AAA9413111) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFE2L1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFE2L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the NFE2L1 nfe2l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFE2L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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