Rabbit anti-Human, Mouse NF-Y beta Polyclonal Antibody | anti-NFYB antibody

NF-Y beta (NF-YB, Nuclear Factor Yb)

Cat. Num. AAA617970

• Gene Names: NFYB; HAP3; CBF-A; CBF-B; NF-YB
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Serum; Purified by delipidation and salt fractionation
• Synonyms: NF-Y beta; Polyclonal Antibody; NF-Y beta (NF-YB; Nuclear Factor Yb); Anti -NF-Y beta (NF-YB; anti-NFYB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes the human and mouse NF-Y (B subunit) protein.
• Purity/Purification: Serum; Purified by delipidation and salt fractionation
• Form/Format: Supplied as a liquid, 0.1% sodium azide. No stabilizing proteins added.

• Applicable Applications for anti-NFYB antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for Immunoprecipitation, Immunoblot, ELISA and Supershift assays.; Dilution: ELISA: 1:10,000; Immunoblot: 1:2000; Super shift: 0.5-1ul/assay.
• Immunogen: A synthetic peptide corresponding to a region near the N-terminus of the human NF-Y (B subunit) protein (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-NFYB antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 5453780
• NCBI GeneID: 4801
• NCBI Accession #: NP_006157.1
• NCBI GenBank Nucleotide #: NM_006166.3
• UniProt Accession #: P25208; Q96IY8; A8K7B9
• Molecular Weight: 22,831 Da
• NCBI Official Full Name: nuclear transcription factor Y subunit beta
• NCBI Official Synonym Full Names: nuclear transcription factor Y, beta
• NCBI Official Symbol: NFYB
• NCBI Official Synonym Symbols: HAP3; CBF-A; CBF-B; NF-YB
• NCBI Protein Information: nuclear transcription factor Y subunit beta; Transcription factor NF-Y, B subunit; CAAT box DNA-binding protein subunit B; nuclear transcription factor Y subunit B; CCAAT-binding transcription factor subunit A
• UniProt Protein Name: Nuclear transcription factor Y subunit beta
• Protein Family: Nuclear transcription factor
• UniProt Gene Name: NFYB
• UniProt Synonym Gene Names: HAP3
• UniProt Entry Name: NFYB_HUMAN

NCBI Description

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Uniprot Description

NFYB: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYB/HAP3 subunit family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q22-q23

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process