SDS-Page
Islet Cell Autoantigen 1 (ICA1) Recombinant Protein | ICA1 recombinant protein
Recombinant Islet Cell Autoantigen 1 (ICA1)
Gene Names
Ica1; 69kDa; ICA69
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Islet Cell Autoantigen 1 (ICA1); Recombinant Islet Cell Autoantigen 1 (ICA1); ICA1 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-MSGHKCYSWE LQDRFAQDKS VVNKMQQKYW ETKQAFIKAT GKKEDEHVVA SDADLDAKLE LFHSIQRTCL DLSKAIVLYQ KRICFLSQEE NELGKFLRSQ GFQDKTRAGK MMQATGKALC FSSQQRLALR NPLCRFHQEV ETFRHRAISD TWLTVNRMEQ YRTEYRGALL WMKDVSQELD PDLYKQMEKF RKVQTQVRLA KKNFDKLKMD VCQKVDLLGA SRCNLLSHML ATYQTTLLHF WEKTSHTMAA IHESFKG
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-MSGHKCYSWE LQDRFAQDKS VVNKMQQKYW ETKQAFIKAT GKKEDEHVVA SDADLDAKLE LFHSIQRTCL DLSKAIVLYQ KRICFLSQEE NELGKFLRSQ GFQDKTRAGK MMQATGKALC FSSQQRLALR NPLCRFHQEV ETFRHRAISD TWLTVNRMEQ YRTEYRGALL WMKDVSQELD PDLYKQMEKF RKVQTQVRLA KKNFDKLKMD VCQKVDLLGA SRCNLLSHML ATYQTTLLHF WEKTSHTMAA IHESFKG
Sequence Length
457
Applicable Applications for ICA1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Met1~Gly257 (Accession # P97411) with two N-terminal Tags, His-tag and S-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Predicted isoelectric point
8.8
Predicted Molecular Mass
35.9kDa as determined by SDS-PAGE reducing conditions.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26&32&37kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
islet cell autoantigen 1 isoform 2
NCBI Official Synonym Full Names
islet cell autoantigen 1
NCBI Official Symbol
Ica1
NCBI Official Synonym Symbols
69kDa; ICA69
NCBI Protein Information
islet cell autoantigen 1; p69; ICAp69; islet cell autoantigen p69; 69 kDa islet cell autoantigen; islet cell autoantigen 1, 69 kDa
UniProt Protein Name
Islet cell autoantigen 1
Protein Family
UniProt Gene Name
Ica1
UniProt Synonym Gene Names
Icap69; ICA69; ICAp69; p69
UniProt Entry Name
ICA69_MOUSE
Uniprot Description
ICA1: May play a role in neurotransmitter secretion.
Protein type: Unknown function
Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; synaptic vesicle membrane; membrane; perinuclear region of cytoplasm; secretory granule membrane; dendrite; cytoplasm; synapse; cytoplasmic vesicle; cell junction; cytosol
Molecular Function: protein domain specific binding
Biological Process: transport; neurotransmitter transport; regulation of protein homodimerization activity; regulation of neurotransmitter secretion
Research Articles on ICA1
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Product Notes
The ICA1 ica1 (Catalog #AAA2012459) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Islet Cell Autoantigen 1 (ICA1) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP) (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the ICA1 ica1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-MSGHKCY SWE LQDRFAQDKS VVNKMQQKYW ETKQAFIKAT GKKEDEHVVA SDADLDAKLE LFHSIQRTCL DLSKAIVLYQ KRICFLSQEE NELGKFLRSQ GFQDKTRAGK MMQATGKALC FSSQQRLALR NPLCRFHQEV ETFRHRAISD TWLTVNRMEQ YRTEYRGALL WMKDVSQELD PDLYKQMEKF RKVQTQVRLA KKNFDKLKMD VCQKVDLLGA SRCNLLSHML ATYQTTLLHF WEKTSHTMAA IHESFKG. It is sometimes possible for the material contained within the vial of "Islet Cell Autoantigen 1 (ICA1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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