Rabbit Neurotrypsin Polyclonal Antibody | anti-PRSS12 antibody

Neurotrypsin Antibody

Cat. Num. AAA150097

• Gene Names: PRSS12; MRT1; BSSP3; BSSP-3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Neurotrypsin Antibody is affinity chromatography purified via peptide column.
• Synonyms: Neurotrypsin; Polyclonal Antibody; Neurotrypsin Antibody; MRT1; BSSP3; BSSP-3; Leydin; protease; serine; 12 (neurotrypsin; motopsin); anti-PRSS12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Neurotrypsin Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 875

• Applicable Applications for anti-PRSS12 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry Paraffin (IHC-P), Immunofluorescence (IF)
• Application Notes: Neurotrypsin antibody can be used for detection of neurotrypsin by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.; ; Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Conjugate: Unconjugated
• Immunogen: Neurotrypsin antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human neurotrypsin.; The immunogen is located within amino acids 110-160 of Neurotrypsin
• Positive Control: MBS151593 - (NIH) Cell Lysate; MBS154323 - Human Brain Tissue Slide
• Preparation and Storage: Neurotrypsin antibody can be stored at 4° for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Immunofluorescence (IF)

(Immunofluorescence of Neurotrypsin in Human Brain cells with Neurotrypsin antibody at 20 μg/mL.)

Western Blot (WB)

(Neurotrypsin AntibodyWestern blot analysis of neurotrypsin in 3T3 cell lysate with neurotrypsin antibody at (A) 0.25 and (B) 0.5 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Neurotrypsin in human brain tissue with Neurotrypsin antibody at 2.5 μg/mL.)

Related Product Information for anti-PRSS12 antibody

Neurotrypsin Antibody: Neurotrypsin is a central nervous system-expressed serine protease whose truncation or absence causes nonsyndromic mental retardation. It is most prominently expressed in structures that are involved in the processing and storage of learned behavior and memory, such as the cerebral cortex, the hippocampus, and amygdala. Evidence suggests that neurotrypsin has multiple functions, including axonal outgrowth, maintaining neuronal plasticity, and arranging the perineuronal environment, partly in coordination with other proteases including tissue plasminogen activator. At least two isoforms of neurotrypsin are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 2661424
• NCBI GeneID: 8492
• UniProt Accession #: P56730; Q9UP16
• Molecular Weight: Predicted: 96 kDa; Observed: 96 kDa
• NCBI Official Full Name: neurotrypsin
• NCBI Official Synonym Full Names: protease, serine, 12 (neurotrypsin, motopsin)
• NCBI Official Symbol: PRSS12
• NCBI Official Synonym Symbols: MRT1; BSSP3; BSSP-3
• NCBI Protein Information: neurotrypsin; leydin; brain-specific serine protease 3
• UniProt Protein Name: Neurotrypsin
• Protein Family: Neurotrypsin
• UniProt Gene Name: PRSS12
• UniProt Entry Name: NETR_HUMAN

NCBI Description

This gene encodes a member of the trypsin family of serine proteases. Studies in mouse suggest that the encoded enzyme may be involved in structural reorganizations associated with learning and memory. The enzyme is also expressed in Leydig cells in the testis, but its function in this tissue is unknown. Defects in this gene are a cause of mental retardation autosomal recessive type 1 (MRT1). [provided by RefSeq, Jul 2010]

Uniprot Description

PRSS12: Plays a role in neuronal plasticity and the proteolytic action may subserve structural reorganizations associated with learning and memory operations. Defects in PRSS12 are the cause of mental retardation autosomal recessive type 1 (MRT1). Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; EC 3.4.21.-; Vesicle; Protease; Secreted

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: axon; dendrite; plasma membrane; extracellular region; terminal button; cytoplasmic vesicle

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; exocytosis; zymogen activation; proteolysis

Disease: Mental Retardation, Autosomal Recessive 1