Rabbit anti-Human, Mouse NeuroD1 Polyclonal Antibody | anti-NeuroD1 antibody

NeuroD1, CT (NEUROD1, BHLHA3, NEUROD, Neurogenic differentiation factor 1, Class A basic helix-loop-helix protein 3) (Biotin)

Cat. Num. AAA6324808

• Gene Names: NEUROD1; BETA2; BHF-1; MODY6; NEUROD; bHLHa3
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: NeuroD1; Polyclonal Antibody; CT (NEUROD1; BHLHA3; NEUROD; Neurogenic differentiation factor 1; Class A basic helix-loop-helix protein 3) (Biotin); NEUROD1; Class A basic helix-loop-helix protein 3; anti-NeuroD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-NeuroD1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1,000; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NeuroD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326~355 amino acids from the C-terminal region of human NeuroD1 protein.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NeuroD1 Antibody (I333) western blot analysis in Y79 cell line,mouse cerebellum and rat brain tissue lysates (35ug/lane).This demonstrates the NeuroD1 antibody detected the NeuroD1 protein (arrow).)

Related Product Information for anti-NeuroD1 antibody

NeuroD1 is a transcriptional activator that acts as a differentiation factor during neurogenesis. It has been demonstrated to bind to the insulin gene E-box. Efficient DNA binding requires dimerization with another basic helix-loop-helix (bHLH) protein. Defects in NEUROD1 are a cause of maturity onset diabetes of the young type VI (MODY6). MODY6 is a form of non-insulin-dependent diabetes mellitus characterized by an autosomal dominant mode of inheritance, onset during young adulthood and a primary defect in insulin secretion.

Product Categories/Family for anti-NeuroD1 antibody

Antibodies; Transcription Factors

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Miyachi, T., et al., Brain Res. Mol. Brain Res. 69(2):223-231 (1999). Malecki, M.T., et al., Nat. Genet. 23(3):323-328 (1999). Acharya, H.R., et al., Biochem. Biophys. Res. Commun. 233(2):459-463 (1997). Yokoyama, M., et al., Brain Res. Mol. Brain Res. 42(1):135-139 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 121114306
• NCBI GeneID: 4760
• NCBI Accession #: NP_002491.2
• NCBI GenBank Nucleotide #: NM_002500.4
• UniProt Accession #: Q13562; O00343; Q13340; Q5U095; Q96TH0; Q99455; Q9UEC8; B2R9I8; F1T0E1
• Molecular Weight: 39,920 Da
• NCBI Official Full Name: neurogenic differentiation factor 1
• NCBI Official Synonym Full Names: neuronal differentiation 1
• NCBI Official Symbol: NEUROD1
• NCBI Official Synonym Symbols: BETA2; BHF-1; MODY6; NEUROD; bHLHa3
• NCBI Protein Information: neurogenic differentiation factor 1; beta-cell E-box transactivator 2; class A basic helix-loop-helix protein 3; neurogenic helix-loop-helix protein NEUROD; basic helix-loop-helix transcription factor
• UniProt Protein Name: Neurogenic differentiation factor 1
• UniProt Gene Name: NEUROD1
• UniProt Synonym Gene Names: BHLHA3; NEUROD; NeuroD; NeuroD1; bHLHa3
• UniProt Entry Name: NDF1_HUMAN

NCBI Description

This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]

Uniprot Description

NEUROD1: Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulates transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor p21Cip1. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. Interacts (via helix-loop-helix motif domain) with EP300 (via C-terminus). Heterodimer with TCF3/E47; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E- box element. Efficient DNA-binding requires dimerization with another bHLH protein. Interacts with RREB1. Interacts with EP300; the interaction is inhibited by NR0B2. Interacts with TCF3; the interaction is inhibited by ID2.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: response to drug; dentate gyrus development; transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; nucleocytoplasmic transport; glucose homeostasis; endocrine pancreas development; anterior/posterior pattern formation; embryonic organ morphogenesis; neurogenesis; insulin secretion; response to glucose stimulus; cerebellum development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of neuron differentiation; positive regulation of cell differentiation; regulation of insulin secretion; negative regulation of JAK-STAT cascade; nitric oxide mediated signal transduction; inner ear development

Disease: Maturity-onset Diabetes Of The Young, Type 6; Diabetes Mellitus, Noninsulin-dependent