Rabbit anti-Human NETO1 Polyclonal Antibody | anti-NETO1 antibody

NETO1 Antibody

Cat. Num. AAA7108459

• Gene Names: NETO1; BCTL1; BTCL1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: NETO1; Polyclonal Antibody; NETO1 Antibody; Neuropilin and tolloid-like protein 1; Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1; BTCL1; anti-NETO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 533

• Applicable Applications for anti-NETO1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IF: 1:50-1:200
• Immunogen: Recombinant Human Neuropilin and tolloid-like protein 1 protein (367-507AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate, 293T whole cell lysate, U87 whole cell lysateAll lanes: NETO1 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61, 18 KDaObserved band size: 61 KDa)

Immunofluorescence (IF)

(Immunofluorescence staining of HepG2 cells with MBS7108459 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Related Product Information for anti-NETO1 antibody

Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory (By similarity).

Product Categories/Family for anti-NETO1 antibody

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 319918840
• NCBI GeneID: 81832
• NCBI Accession #: NP_001188394.1
• NCBI GenBank Nucleotide #: NM_001201465.2
• UniProt Accession #: Q8TDF5; Q86W85; Q8ND78; Q8TDF4
• Molecular Weight: 60,060 Da
• NCBI Official Full Name: neuropilin and tolloid-like protein 1 isoform 3
• NCBI Official Synonym Full Names: neuropilin and tolloid like 1
• NCBI Official Symbol: NETO1
• NCBI Official Synonym Symbols: BCTL1; BTCL1
• NCBI Protein Information: neuropilin and tolloid-like protein 1
• UniProt Protein Name: Neuropilin and tolloid-like protein 1
• Protein Family: Neuropilin and tolloid-like protein
• UniProt Gene Name: NETO1
• UniProt Synonym Gene Names: BTCL1

NCBI Description

This gene encodes a transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. This protein is thought to play a critical role in spatial learning and memory by regulating the function of synaptic N-methyl-D-aspartic acid receptor complexes in the hippocampus. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2017]

Uniprot Description

Involved in the development and/or maintenance of neuronal circuitry. Accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. Regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory ().