NETO1 / BTCL1 recombinant protein

Recombinant Human NETO1 / BTCL1 Protein (His tag)

Cat. Num. AAA2545744

• Gene Names: NETO1; BCTL1; BTCL1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: NETO1 / BTCL1; Recombinant Human NETO1 / BTCL1 Protein (His tag); BCTL1; BTCL1; NETO1 / BTCL1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 533

• Application Notes: The secreted recombinant human NETO1 consists of 333 amino acids and has a calculated molecular mass of 38 kDa. It migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Thr 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for NETO1 / BTCL1 recombinant protein

Background: Neuropilin tolloid-like 1 (NETO1), a complement C1r/C1s, Uegf, Bmp1 (CUB) domain-containing transmembrane protein, is a novel component of the NMDAR complex critical for maintaining the abundance of NR2A-containing NMDARs in the postsynaptic density. The N-methyl-D-aspartate receptor (NMDAR), a major excitatory ligand-gated ion channel in the central nervous system (CNS), is a principal mediator of synaptic plasticity. Both NETO1 and NETO2 share an identical and unique domain structure thus representing a novel subfamily of CUB- and LDLa-containing proteins. The cytoplasmic domains of NETO1 and NETO2 are not homologous to other known protein sequences but contain a conserved FXNPXY-like motif, which is essential for the internalization of clathrin coated pits during endocytosis or alternatively, may be implicated in intracellular signaling pathways. NETO1 and NETO2, have marked effects on receptor properties, increasing further the potential diversity of Kainate receptors (KARs) functional properties. NETO1 involves in the development and/or maintenance of neuronal circuitry. NETO1 regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.

Description: A DNA sequence encoding the human NETO1 isoform 3 (NP_620416.1) extracellular domain (Met 1-Thr 344) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 20373181
• NCBI GeneID: 81832
• NCBI Accession #: NP_620416.1
• NCBI GenBank Nucleotide #: NM_138966.3
• UniProt Accession #: Q8TDF5; Q86W85; Q8ND78; Q8TDF4
• Molecular Weight: 60,060 Da
• NCBI Official Full Name: neuropilin and tolloid-like protein 1 isoform 3
• NCBI Official Synonym Full Names: neuropilin and tolloid like 1
• NCBI Official Symbol: NETO1
• NCBI Official Synonym Symbols: BCTL1; BTCL1
• NCBI Protein Information: neuropilin and tolloid-like protein 1
• UniProt Protein Name: Neuropilin and tolloid-like protein 1
• UniProt Gene Name: NETO1
• UniProt Synonym Gene Names: BTCL1

NCBI Description

This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. A similar gene in mice encodes a protein that plays a critical role in spatial learning and memory by regulating the function of synaptic N-methyl-D-aspartic acid receptor complexes in the hippocampus. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

NETO1: a transmembrane protein and component of the N-methyl-D-aspartate receptor (NMDAR) complex. Required to maintain the normal level of NMDAR subunits at synapses. The NMDAR complex is a major excitatory ligand-gated ion channel in the central nervous system and a major mediator of synaptic plasticity. Important in the development and/or maintenance of neuronal circuitry. Neto1-deficient mice have a dramatic decrease in the number of NMDA receptors at synapses, impairing learning and synaptic plasticity. Three isoforms of the human protein are produced by alternative splicing. Isoforms 1 and 2 are retina-specific whereas isoform 3 is found in retina as well as at lower levels in adult and fetal brain.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q22.3

Cellular Component: excitatory synapse; postsynaptic density

Biological Process: memory; regulation of long-term neuronal synaptic plasticity; visual learning