Western Blot (WB)
(Western Blot analysis of extracts of various cells, using RPP25 antibody.)
Rabbit anti-Human RPP25 Polyclonal Antibody | anti-RPP25 antibody
RPP25 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
RPP25; Polyclonal Antibody; RPP25 Polyclonal Antibody; Ribonuclease P protein subunit p25; RNase P protein subunit p25; anti-RPP25 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
RPP25 polyclonal antibody detects endogenous levels of RPP25 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
199
Applicable Applications for anti-RPP25 antibody
Western Blot (WB)
Application Notes
WB: 1:200-1:500
Immunogen
Recombinant protein of human RPP25.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using RPP25 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using RPP25 antibody.)
Related Product Information for anti-RPP25 antibody
Ribonuclease P (RNase P) and Ribonuclease MRP (RNase MRP) are small nuclear ribonucleoproteins (snRNPs) that act on RNA substrates in vitro. RNase P and RNase MRP, which accumulate in the nucleolus, have a similar RNA component and also have several protein subunits in common. RNase P, which consists of a complex of an RNA species, POP1, POP5, and at least 7 Rpps, removes the 5' leader sequences from precursor tRNA molecules. Rpp25 (ribonuclease P/MRP 25 kDa subunit) is a 199 amino acid nuclear protein that belongs to the histone-like Alba family and functions as a component of nuclear RNase P and RNase MRP ribonucleoproteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 21kDa
NCBI Official Full Name
ribonuclease P protein subunit p25
NCBI Official Synonym Full Names
ribonuclease P and MRP subunit p25
NCBI Official Symbol
RPP25
NCBI Protein Information
ribonuclease P protein subunit p25
UniProt Protein Name
Ribonuclease P protein subunit p25
Protein Family
UniProt Gene Name
RPP25
UniProt Synonym Gene Names
RNase P protein subunit p25
UniProt Entry Name
RPP25_HUMAN
Uniprot Description
Function: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. This subunit binds to RNA.
Catalytic activity: Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Subunit structure: Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a RNA moiety and at least 8 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38 and RPP40. Interacts with the P3 domain of RNase MRP complex. RNase MRP consists of a RNA moiety and at least 9 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38, RPP40, POP5 and RPP21. Ref.5
Subcellular location: Nucleus Ref.1.
Sequence similarities: Belongs to the histone-like Alba family.
Research Articles on RPP25
Similar Products
Product Notes
The RPP25 rpp25 (Catalog #AAA3009364) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPP25 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPP25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200-1:500. Researchers should empirically determine the suitability of the RPP25 rpp25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPP25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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