Rabbit anti-Human NELFB Polyclonal Antibody | anti-NELFB antibody

NELFB (Negative Elongation Factor B, NELF-B, Cofactor of BRCA1, COBRA1, KIAA1182, RP13-122B23.3)

Cat. Num. AAA6012150

• Gene Names: NELFB; COBRA1; NELF-B; RP13-122B23.3
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NELFB; Polyclonal Antibody; NELFB (Negative Elongation Factor B; NELF-B; Cofactor of BRCA1; COBRA1; KIAA1182; RP13-122B23.3); Anti -NELFB (Negative Elongation Factor B; anti-NELFB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RP13-122B23.3.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL

• Applicable Applications for anti-NELFB antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human RP13-122B23.3, aa1-475 (AAH11892.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RP13-122B23.3 rabbit polyclonal antibody. Western Blot analysis of RP13-122B23.3 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of COBRA1 expression in transfected 293T cell line by COBRA1 polyclonal antibody. Lane 1: RP13-122B23.3 transfected lysate (53.9kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-NELFB antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 20070260
• NCBI GeneID: 25920
• NCBI Accession #: NP_056271.2
• NCBI GenBank Nucleotide #: NM_015456.3
• UniProt Accession #: Q8WX92; Q96EW5; Q9H9R4; Q9ULN8; Q9Y3W0; A2BFA3
• Molecular Weight: 65,697 Da
• NCBI Official Full Name: negative elongation factor B
• NCBI Official Synonym Full Names: negative elongation factor complex member B
• NCBI Official Symbol: NELFB
• NCBI Official Synonym Symbols: COBRA1; NELF-B; RP13-122B23.3
• NCBI Protein Information: negative elongation factor B; cofactor of BRCA1; negative elongation factor protein B
• UniProt Protein Name: Negative elongation factor B
• UniProt Gene Name: NELFB
• UniProt Synonym Gene Names: COBRA1; KIAA1182; NELF-B
• UniProt Entry Name: NELFB_HUMAN

NCBI Description

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

Uniprot Description

COBRA1: a subunit of the multisubunit transcription elongation factor NELF (negative elongation factor). May be involved in transcriptional pausing of RNA polymerase II (RNAPII). Interacts with the first BRCT repeat of BRCA1.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent