Rabbit anti-Human NELFB Polyclonal Antibody | anti-NELFB antibody

NELFB (Negative Elongation Factor B, NELF-B, Cofactor of BRCA1, COBRA1, KIAA1182, RP13-122B23.3) (FITC)

Cat. Num. AAA6392840

• Gene Names: NELFB; COBRA1; NELF-B
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NELFB; Polyclonal Antibody; NELFB (Negative Elongation Factor B; NELF-B; Cofactor of BRCA1; COBRA1; KIAA1182; RP13-122B23.3) (FITC); anti-NELFB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RP13-122B23.3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 2291

• Applicable Applications for anti-NELFB antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human RP13-122B23.3, aa1-475 (AAH11892.1).
• Immunogen Sequence: MPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVSYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPAPAPL
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RP13-122B23.3 rabbit polyclonal antibody. Western Blot analysis of RP13-122B23.3 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of COBRA1 expression in transfected 293T cell line by COBRA1 polyclonal antibody. Lane 1: RP13-122B23.3 transfected lysate (53.9kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-NELFB antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 15080249
• NCBI GeneID: 25920
• NCBI Official Full Name: Homo sapiens cofactor of BRCA1, mRNA
• NCBI Official Synonym Full Names: negative elongation factor complex member B
• NCBI Official Symbol: NELFB
• NCBI Official Synonym Symbols: COBRA1; NELF-B
• NCBI Protein Information: negative elongation factor B

NCBI Description

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]