Rabbit anti-Human COBRA1 Polyclonal Antibody | anti-COBRA1 antibody

COBRA1, ID (COBRA1, KIAA1182, NELFB, Negative elongation factor B, Cofactor of BRCA1) (MaxLight 490)

Cat. Num. AAA6287477

• Gene Names: NELFB; COBRA1; NELF-B
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: COBRA1; Polyclonal Antibody; ID (COBRA1; KIAA1182; NELFB; Negative elongation factor B; Cofactor of BRCA1) (MaxLight 490); Cofactor of BRCA1; anti-COBRA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COBRA1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-COBRA1 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 256-285 in the central region of human COBRA1. Species sequence homology: mouse
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS6006204.This demonstrates that MBS6006204 detected the COBRA1 protein (arrow).)

Related Product Information for anti-COBRA1 antibody

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).

Product Categories/Family for anti-COBRA1 antibody

Antibodies; KIAA; orf

References

1. Sun, J., et al. J. Cell. Biochem. 103(6):1798-1807(2008). 2. Sun, J., et al. J. Steroid Biochem. Mol. Biol. 107 (3-5), 131-139 (2007). 3. Wu, C., et al. Proteomics 7(11):1775-1785(2007). 4. Narita, T., et al. Mol. Cell 26(3):349-365(2007). 5. Aiyar, S.E., et al. Int. J. Biol. Sci. 3(7):486-492(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 20070260
• NCBI GeneID: 25920
• NCBI Accession #: NP_056271.2
• NCBI GenBank Nucleotide #: NM_015456.3
• UniProt Accession #: Q8WX92; Q96EW5; Q9H9R4; Q9ULN8; Q9Y3W0; A2BFA3
• Molecular Weight: 65,697 Da
• NCBI Official Full Name: negative elongation factor B
• NCBI Official Synonym Full Names: negative elongation factor complex member B
• NCBI Official Symbol: NELFB
• NCBI Official Synonym Symbols: COBRA1; NELF-B
• NCBI Protein Information: negative elongation factor B; cofactor of BRCA1; negative elongation factor protein B
• UniProt Protein Name: Negative elongation factor B
• UniProt Gene Name: NELFB
• UniProt Synonym Gene Names: COBRA1; KIAA1182; NELF-B
• UniProt Entry Name: NELFB_HUMAN

Uniprot Description

COBRA1: a subunit of the multisubunit transcription elongation factor NELF (negative elongation factor). May be involved in transcriptional pausing of RNA polymerase II (RNAPII). Interacts with the first BRCT repeat of BRCA1.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent