Immunohistochemistry (IHC)
(MBS9603706 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse SPAG4L Polyclonal Antibody | anti-SPAG4L antibody
SPAG4L Antibody
Gene Names
SUN5; SPAG4L; SPGF16; TSARG4; dJ726C3.1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SPAG4L; Polyclonal Antibody; SPAG4L Antibody; dJ726C3.1; MGC33594; SPAG4-like protein; Sperm associated antigen 4 like protein; SUN domain-containing protein 5; SUN5; SUN5 Sad1 and UNC84 domain containing 5; testis and spermatogenesis related gene 4; Testis and spermatogenesis related gene 4 protein; TSARG4; anti-SPAG4L antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SPAG4L antibody detects endogenous levels of SPAG4L.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
379
Applicable Applications for anti-SPAG4L antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human SPAG4L.
Subcellular Location
Nuclear; Integral Component of Membrane
Tissue Specificity
Sperm (at protein level) (PubMed:27640305). Widely expressed (PubMed:12621555). Conflictingly shown to be specifically expressed in testis (PubMed:21711156).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603706 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603706 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using SPAG4L antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using SPAG4L antibody.)
Related Product Information for anti-SPAG4L antibody
Function: Plays an essential role in anchoring sperm head to the tail. Is responsible for the attachment of the coupling apparatus to the sperm nuclear envelope.
Subunit Structure: Probable homotrimer. Interacts with DNAJB13.
Post-translational Modifications: Highly glycosylated in the Golgi apparatus during spermiogenesis.
Subunit Structure: Probable homotrimer. Interacts with DNAJB13.
Post-translational Modifications: Highly glycosylated in the Golgi apparatus during spermiogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 43 kDa
Predicted: 44 kDa
Predicted: 44 kDa
NCBI Official Full Name
SUN domain-containing protein 5
NCBI Official Synonym Full Names
Sad1 and UNC84 domain containing 5
NCBI Official Symbol
SUN5
NCBI Official Synonym Symbols
SPAG4L; SPGF16; TSARG4; dJ726C3.1
NCBI Protein Information
SUN domain-containing protein 5
UniProt Protein Name
SUN domain-containing protein 5
UniProt Gene Name
SUN5
UniProt Synonym Gene Names
SPAG4L; TSARG4
NCBI Description
The protein encoded by this gene appears to play a role in the meiotic stage of spermatogenesis. The encoded protein localizes to the junction between the sperm head and body and may be involved in nuclear envelope reconstitution and nuclear migration. Mutations in this gene have been implicated in acephalic spermatozoa syndrome. [provided by RefSeq, May 2017]
Uniprot Description
Plays an essential role in anchoring sperm head to the tail. Is responsible for the attachment of the coupling apparatus to the sperm nuclear envelope.
Research Articles on SPAG4L
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Product Notes
The SPAG4L sun5 (Catalog #AAA9603706) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPAG4L Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPAG4L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SPAG4L sun5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPAG4L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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