Immunohistochemistry (IHC)
(Anti-PAICS / ADE2 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Mouse anti-Human PAICS / ADE2 Monoclonal Antibody | anti-PAICS antibody
Mouse Monoclonal [clone 1B4] (IgG2b) to Human PAICS / ADE2
Usage: Concentration: 1.00 mg/ml (Lot dependent)
Immunohistochemistry (IHC)
(Anti-PAICS / ADE2 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Anti-PAICS / ADE2 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunofluorescence (IF)
(Anti-PAICS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAICS.)
Immunofluorescence (IF)
(Anti-PAICS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAICS.)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PAICS antibody, and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PAICS antibody, and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(Flow cytometry of Jurkat cells, using anti-PAICS antibody, (Red), compared to a nonspecific negative control antibody, (Blue).)
Flow Cytometry (FC/FACS)
(Flow cytometry of Jurkat cells, using anti-PAICS antibody, (Red), compared to a nonspecific negative control antibody, (Blue).)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a bifunctional enzyme containing phosphoribosylaminoimidazole carboxylase activity in its N-terminal region and phosphoribosylaminoimidazole succinocarboxamide synthetase in its C-terminal region. It catalyzes steps 6 and 7 of purine biosynthesis. The gene is closely linked and divergently transcribed with a locus that encodes an enzyme in the same pathway, and transcription of the two genes is coordinately regulated. The human genome contains several pseudogenes of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PAICS: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; EC 6.3.2.6; Nucleotide Metabolism - purine; EC 4.1.1.21; Lyase
Chromosomal Location of Human Ortholog: 4q12
Cellular Component: membrane; cytosol
Molecular Function: phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; identical protein binding; protein binding; phosphoribosylaminoimidazole carboxylase activity; ATP binding
Biological Process: purine ribonucleoside monophosphate biosynthetic process; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; 'de novo' IMP biosynthetic process
Research Articles on PAICS
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Product Notes
The PAICS paics (Catalog #AAA249092) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 1B4] (IgG2b) to Human PAICS / ADE2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAICS / ADE2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS). Flo (1:100), IF (1:100), IHC-P (10 ug/ml), WB (1:2000) Usage: Concentration: 1.00 mg/ml (Lot dependent). Researchers should empirically determine the suitability of the PAICS paics for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAICS / ADE2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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