Rabbit anti-Human NEIL1 Polyclonal Antibody | anti-NEIL1 antibody

NEIL1, ID (NEIL1, Endonuclease 8-like 1, DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, Nei homolog 1, Nei-like protein 1)

Cat. Num. AAA649896

• Gene Names: NEIL1; FPG1; NEI1; hFPG1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: NEIL1; Polyclonal Antibody; ID (NEIL1; Endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; Nei-like protein 1); Anti -NEIL1; anti-NEIL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NEIL1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100
• Immunogen: NEIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137~167 amino acids from the Central region of human NEIL1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NEIL1 Antibody (Center) in A2058 cell line lysates (35ug/lane). NEIL1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-NEIL1 antibody

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).

Product Categories/Family for anti-NEIL1 antibody

Antibodies; Abs to Enzymes; Endonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 14790078
• NCBI GeneID: 79661
• UniProt Accession #: Q96FI4; Q6ZRA7; Q86XW7; Q9H6C3; D3DW75
• Molecular Weight: 43,684 Da
• NCBI Official Full Name: NEIL1 protein
• NCBI Official Synonym Full Names: nei endonuclease VIII-like 1 (E. coli)
• NCBI Official Symbol: NEIL1
• NCBI Official Synonym Symbols: FPG1; NEI1; hFPG1
• NCBI Protein Information: endonuclease 8-like 1; NEH1; nei homolog 1; endonuclease VIII; nei-like protein 1; endonuclease VIII-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1
• UniProt Protein Name: Endonuclease 8-like 1
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL1
• UniProt Synonym Gene Names: NEH1
• UniProt Entry Name: NEIL1_HUMAN

NCBI Description

This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

Function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Ref.1 Ref.7 Ref.8 Ref.9

Catalytic activity: Removes damaged bases from DNA, leaving an abasic site.The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Nucleus. Chromosome. Note: During mitosis, associates with centrosomes and condensed chromatin. Ref.10

Tissue specificity: Ubiquitous. Ref.8

Induction: Up-regulated during S-phase. Ref.8

Sequence similarities: Belongs to the FPG family.

RNA editing: Edited at position 242.The edited form removes thymine glycol from duplex DNA 30 times more slowly than the form encoded in the genome, whereas editing enhances repair of the guanidinohydantoin lesion by NEIL1. The recoding site is a preferred editing site for the RNA editing adenosine deaminase ADAR1. Ref.11

Sequence caution: The sequence AK128372 differs from that shown. Reason: Erroneous CDS prediction.