Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Rabbit NEK7 Polyclonal Antibody | anti-NEK7 antibody

NEK7 Polyclonal Antibody

Reactivity
Human, Mouse, Rat, Chicken, Pig, Cow, Rabbit, Sheep
Applications
Immunohistochemistry
Purity
affinity purified by Protein A
Synonyms
NEK7; Polyclonal Antibody; NEK7 Polyclonal Antibody; NEK7_HUMAN; Never in mitosis A-related kinase 7; Never in mitosis gene a-related kinase 7; NIMA (never in mitosis gene a) related kinase 7; NIMA (never in mitosis gene a)-related expressed kinase 7; NimA related protein kinase 7; NIMA-related kinase 7; NimA-related protein kinase 7; Serine/threonine-protein kinase Nek7; anti-NEK7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Pig, Cow, Rabbit, Sheep
Clonality
Polyclonal
Specificity
NEK7 Polyclonal Antibody detects endogenous levels of NEK7 protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-NEK7 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-500
Immunogen
KLH conjugated synthetic peptide derived from human NEK7:101-200/302
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C,)

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C,)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C,)

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C,)
Related Product Information for anti-NEK7 antibody
Tissue specificity: Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,551 Da
NCBI Official Full Name
serine/threonine-protein kinase Nek7
NCBI Official Synonym Full Names
NIMA-related kinase 7
NCBI Official Symbol
NEK7
NCBI Protein Information
serine/threonine-protein kinase Nek7; nimA-related protein kinase 7; never in mitosis A-related kinase 7; NIMA (never in mitosis gene a)-related kinase 7
UniProt Protein Name
Serine/threonine-protein kinase Nek7
UniProt Gene Name
NEK7
UniProt Synonym Gene Names
NimA-related protein kinase 7
UniProt Entry Name
NEK7_HUMAN

NCBI Description

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002]

Uniprot Description

NEK7: a protein kinase of the NEK family. Phosphorylates and activates p70S6 kinase.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; EC 2.7.11.1; Other group; NEK family

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: nucleoplasm; spindle pole; microtubule; cytoplasm; microtubule organizing center

Molecular Function: protein serine/threonine kinase activity; metal ion binding; ATP binding

Biological Process: regulation of mitotic cell cycle; cytokinesis; regulation of signal transduction; spindle assembly; protein amino acid phosphorylation

Research Articles on NEK7

Similar Products

Product Notes

The NEK7 nek7 (Catalog #AAA3015526) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEK7 Polyclonal Antibody reacts with Human, Mouse, Rat, Chicken, Pig, Cow, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's NEK7 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-500. Researchers should empirically determine the suitability of the NEK7 nek7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.