NEK7 recombinant protein

NEK7 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8120843

• Purity: >95% as determined by SDS-PAGE
• Synonyms: NEK7; NEK7 Protein; Human; Recombinant (His & GST Tag); Human NEK7 Protein (His & GST Tag); NIMA-related kinase 7; NEK7 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.5, 0.5mM Reduced Glutathione, 0.5mM PMSF. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard
• Sequence: Met1-Ser302

• Species: Human
• Activity: No Kinase Activity
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human NEK7 (NP_598001.1)(Met1-Ser 302) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NEK7 recombinant protein

Background: NIMA (never in mitosis gene a)-related kinase 7, NEK7 belongs to the NIMA subfamily, NEK Ser/Thr protein kinase family, protein kinase superfamily. NEKs (NIMA-related kinases) are mammalian serine/threonine (Ser/Thr) protein kinases structurally related to Aspergillus NIMA (Never in Mitosis, gene A), which plays essential roles in mitotic signaling. NEKs share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying length. NEKs are commonly referred to as mitotic kinases, although a definitive in vivo verification of this definition is largely missing. Reduction in the activity of NEK7 or its close paralog, NEK6, has previously been shown to arrest cells in mitosis, mainly at metaphase. NEK7 is a regulator of cell division, and reveal it as an essential component for mammalian growth and survival. The intimate connection between tetraploidy, aneuploidy, and cancer development suggests that NEK7 deregulation can induce oncogenesis. The endogenous NEK7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective NEK7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells.

References

Belham C, et al. (2003) A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases. J Biol Chem. 278(37): 34897-909.Minoguchi S, et al.. (2003) Differential control of the NIMA-related kinases, Nek6 and Nek7, by serum stimulation. Biochem Biophys Res Commun. 301(4): 899-906.Yissachar N, et al. (2006) Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression. FEBS Lett. 580(27): 6489-95.Salem H, et al. (2010) Nek7 kinase targeting leads to early mortality, cytokinesis disturbance and polyploidy. Oncogene. 29(28): 4046-57.

NCBI and Uniprot Product Information

• NCBI GI #: 19424132
• NCBI GeneID: 140609
• NCBI Accession #: NP_598001.1
• NCBI GenBank Nucleotide #: NM_133494.2
• UniProt Accession #: Q8TDX7; A6NGT8
• Molecular Weight: 34,551 Da
• NCBI Official Full Name: serine/threonine-protein kinase Nek7
• NCBI Official Synonym Full Names: NIMA-related kinase 7
• NCBI Official Symbol: NEK7
• NCBI Protein Information: serine/threonine-protein kinase Nek7; nimA-related protein kinase 7; never in mitosis A-related kinase 7; NIMA (never in mitosis gene a)-related kinase 7
• UniProt Protein Name: Serine/threonine-protein kinase Nek7
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: NEK7
• UniProt Synonym Gene Names: NimA-related protein kinase 7
• UniProt Entry Name: NEK7_HUMAN

NCBI Description

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002]

Uniprot Description

NEK7: a protein kinase of the NEK family. Phosphorylates and activates p70S6 kinase.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; EC 2.7.11.1; Other group; NEK family

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: nucleoplasm; spindle pole; microtubule; cytoplasm; microtubule organizing center

Molecular Function: protein serine/threonine kinase activity; metal ion binding; ATP binding

Biological Process: regulation of mitotic cell cycle; cytokinesis; regulation of signal transduction; spindle assembly; protein amino acid phosphorylation