Rabbit anti-Human NEIL3 Polyclonal Antibody | anti-NEIL3 antibody

NEIL3, ID (NEIL3, Endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3) (PE)

Cat. Num. AAA6324572

• Gene Names: NEIL3; FGP2; FPG2; NEI3; hFPG2; hNEI3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NEIL3; Polyclonal Antibody; ID (NEIL3; Endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Endonuclease VIII-like 3; Nei-like protein 3) (PE); Nei-like protein 3; anti-NEIL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NEIL3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NEIL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-320 amino acids from the Central region of human NEIL3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NEIL3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NEIL3 antibody detected the NEIL3 protein (arrow).)

Related Product Information for anti-NEIL3 antibody

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).

Product Categories/Family for anti-NEIL3 antibody

Antibodies; Enzymes; Endonuclease

References

Krokeide, S.Z., et al. Protein Expr. Purif. 65(2):160-164(2009). Takao, M., et al. Genes Cells 14(2):261-270(2009). Dallosso, A.R., et al. Gut 57(9):1252-1255(2008). Bethke, L., et al. J. Natl. Cancer Inst. 100(4):270-276(2008). Newton-Cheh, C., et al. BMC Med. Genet. 8 SUPPL 1, S7 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 157388969
• NCBI GeneID: 55247
• NCBI Accession #: NP_060718.2
• NCBI GenBank Nucleotide #: NM_018248.2
• UniProt Accession #: Q8TAT5; Q2PPJ3; Q8NG51; Q9NV95
• Molecular Weight: 67,769 Da
• NCBI Official Full Name: endonuclease 8-like 3
• NCBI Official Synonym Full Names: nei endonuclease VIII-like 3 (E. coli)
• NCBI Official Symbol: NEIL3
• NCBI Official Synonym Symbols: FGP2; FPG2; NEI3; hFPG2; hNEI3
• NCBI Protein Information: endonuclease 8-like 3; nei-like protein 3; DNA glycosylase FPG2; DNA glycosylase hFPG2; endonuclease VIII-like 3; DNA glycosylase/AP lyase Neil3
• UniProt Protein Name: Endonuclease 8-like 3
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL3
• UniProt Entry Name: NEIL3_HUMAN

NCBI Description

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]

Uniprot Description

NEIL3: Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single- stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected. Belongs to the FPG family.

Protein type: EC 4.2.99.18; DNA-binding

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: nucleus

Molecular Function: DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; double-stranded DNA binding; DNA N-glycosylase activity; damaged DNA binding; bubble DNA binding; single-stranded DNA binding

Biological Process: nucleotide-excision repair; base-excision repair; DNA catabolic process, endonucleolytic