PTGDS recombinant protein

PTGDS, 23-190aa, Human, His tag, E Coli

Cat. Num. AAA203783

• Gene Names: PTGDS; PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PTGDS; 23-190aa; Human; His tag; E Coli; Prostaglandin-H2 D-isomerase; L-PGDS; LPGDS; PDS; PGD2; PGDS; PGDS2; PTGDS recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 1mM EDTA, 0.1M NaCl
• Concentration: 1 mg/ml (determined by BCA assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAPEAQVSVQ PNFQQDKFLG RWFSAGLASN SSWLREKKAA LSMCKSVVAP ATDGGLNLTS TFLRKNQCET RTMLLQPAGS LGSYSYRSPH WGSTYSVSVV ETDYDQYALL YSQGSKGPGE DFRMATLYSR TQTPRAELKE KFTAFCKAQG FTEDTIVFLP QTDKCMTEQ
• Sequence Length: 190

• Applicable Applications for PTGDS recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PTGDS recombinant protein

Prostaglandin-H2 D-isomerase, also known as PTGDS, is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2). It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. PTGDS is the most abundant protein in the cerebral spinal fluid and recent evidence suggests that PTGDS acts as a beta-amyloid chaperone and may have a role in the deposition of Ab plaques in Alzheimer's disease. Recombinant human PTGDS protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PTGDS recombinant protein

Signal Transduction

References

Hoffmann A., et al. (1993) J Neurochem. 61 (2):451-6.; Giacomelli S., et al. (1996) Biochem Biophys Acta. 1310 (3):269-76.

NCBI and Uniprot Product Information

• NCBI GI #: 32171249
• NCBI GeneID: 5730
• NCBI Accession #: NP_000945
• NCBI GenBank Nucleotide #: NM_000954.5
• UniProt Accession #: P41222; Q5SQ10; Q7M4P3; Q9UC22; Q9UCC9; Q9UCD9; B2R727
• Molecular Weight: 20.9 kDa (189aa), confirmed by MALDI-TOF
• NCBI Official Full Name: prostaglandin-H2 D-isomerase
• NCBI Official Synonym Full Names: prostaglandin D2 synthase
• NCBI Official Symbol: PTGDS
• NCBI Official Synonym Symbols: PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• NCBI Protein Information: prostaglandin-H2 D-isomerase
• UniProt Protein Name: Prostaglandin-H2 D-isomerase
• Protein Family: Prostaglandin-H2 D-isomerase
• UniProt Gene Name: PTGDS
• UniProt Synonym Gene Names: PDS; PGD2 synthase; PGDS; PGDS2

NCBI Description

The protein encoded by this gene is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2). PGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. This gene is preferentially expressed in brain. Studies with transgenic mice overexpressing this gene suggest that this gene may be also involved in the regulation of non-rapid eye movement sleep. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.