Western Blot (WB)
((0. 05ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat NEDD8 conjugating enzyme Polyclonal Antibody | anti-UBE2F antibody
Goat anti-NEDD8 conjugating enzyme (N Terminus) Antibody
Gene Names
UBE2F; NCE2
Reactivity
Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
NEDD8 conjugating enzyme; Polyclonal Antibody; Goat anti-NEDD8 conjugating enzyme (N Terminus) Antibody; Goat Anti-UBE2F/NEDD8-conjugating enzyme Antibody; UBE2F; ubiquitin-conjugating enzyme E2F (putative); MGC18120; NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; NEDD8-conjugating enzyme UBE2F; OTTHUMP00000202749; OTTHUMP00000202755; OTTH; UBE2F antibody; ubiquitin-conjugating enzyme E2F (putative) antibody; MGC18120 antibody; NCE2 antibody; NEDD8 carrier protein UBE2F antibody; NEDD8 conjugating enzyme antibody; NEDD8 protein ligase UBE2F antibody; NEDD8-conjugating enzyme 2 antibody; NEDD8-conjugating enzyme UBE2F antibody; OTTHUMP00000202755 antibody; OTTH antibody; NEDD8 conjugating enzyme (N Terminus); anti-UBE2F antibody
Host
Goat
Reactivity
Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SKLKRDDGLKGSRT
Sequence Length
185
Applicable Applications for anti-UBE2F antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 22kDa band observed in Rat Pancreas lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and 18.6kDa according to Rat NP_001008382.1). Recommended concentration: 0. 05-0.2ug/ml.
Western Blot: Approx 22kDa band observed in Rat Pancreas lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and 18.6kDa according to Rat NP_001008382.1). Recommended concentration: 0. 05-0.2ug/ml.
Immunogen
Peptide with sequence SKLKRDDGLKGSRT-C, from the N Terminus of the protein sequence according to NP_542409.1.
Epitope
N Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0. 05ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 05ug/ml) staining of Rat Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,158 Da
NCBI Official Full Name
NEDD8-conjugating enzyme UBE2F isoform 1
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 F (putative)
NCBI Official Symbol
UBE2F
NCBI Official Synonym Symbols
NCE2
NCBI Protein Information
NEDD8-conjugating enzyme UBE2F
UniProt Protein Name
NEDD8-conjugating enzyme UBE2F
Protein Family
UniProt Gene Name
UBE2F
UniProt Synonym Gene Names
NCE2
UniProt Entry Name
UBE2F_HUMAN
Uniprot Description
UBE2F: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.19; Ligase; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: cytoplasm; nucleus
Molecular Function: ATP binding; ligase activity; NEDD8 ligase activity; protein binding; ubiquitin protein ligase binding
Biological Process: postreplication repair; protein neddylation
Research Articles on UBE2F
Similar Products
Product Notes
The UBE2F ube2f (Catalog #AAA422582) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NEDD8 conjugating enzyme (N Terminus) Antibody reacts with Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD8 conjugating enzyme can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 22kDa band observed in Rat Pancreas lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and 18.6kDa according to Rat NP_001008382.1). Recommended concentration: 0. 05-0.2ug/ml. Researchers should empirically determine the suitability of the UBE2F ube2f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SKLKRDDGLK GSRT. It is sometimes possible for the material contained within the vial of "NEDD8 conjugating enzyme, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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